Development of novel diagnostic methods for anaphylactic shock based on gene expression

仅根据尸检结果确定过敏性休克为死因往往会遇到困难。因此，在本研究中，我们尝试利用小鼠模型建立过敏性休克的新诊断方法。以青霉素V为抗原激发小鼠过敏性休克，从死亡小鼠各器官中提取总RNA。提取的RNA进行实时定量RT-PCR，分析细胞因子、趋化因子、免疫调节分子、生长因子、转录因子和信号转导等基因表达的变化，以确定过敏性休克后特异性基因表达的变化。但目前尚未发现过敏性休克特异性基因表达的变化。

Specifying an anaphylactic shock as the cause of death only based on the autopsy findings often encounters difficulties. So, in this research, we tried development of the novel diagnostic method for anaphylactic shock using the mouse model. Anaphylactic shock was induced by challenge of penicillin V as an antigen, and total RNA was extracted from the various organs of mice died. The extracted RNA was subjected to real-time RT-PCR for analyses of the gene expression of cytokines, chemokines, the molecules for immune regulation, growth factors, transcription factors and signal transducers to identify the changes in specific gene expression anaphylactic shock. However, at present, we did not find the changes of gene expression specific for anaphylactic shock.