HPLC-coupled electrospray ionization time of flight mass spectrometer

在面向化学生物学、分子药理学和药物传递的研究项目背景下，该仪器可以对粗合成样品进行常规实时（即多个用户立即访问）和准确的色谱和质谱分析，即在纯化之前，以及对纯化样品进行精确的质量测定。原油样品的分析是在纯化前确定所需产物的存在以及确定可能的副反应的关键步骤。与慕尼黑路德维希-马克西米利安大学化学和药学院现有的其他液相色谱联用质谱仪器相比，新仪器应适用于较大的合成分子，即m/z高达20,000 Da。典型的样品包括针对药学系新研究的大型合成折叠物，以及大肽、小化学合成蛋白质、折叠物-肽和折叠物-蛋白质共价加合物，以及复杂的脂质/聚合物/核酸偶联物。虽然对这种分析的需求只是分散在整个化学和药学学院，但许多研究小组都期望准时进行分析，但在2017年9月LMU新主席上任后，这种需求变得非常迫切。因此，该工具将被这一群体大量使用，同时对整个学院开放。

In the context of research programs oriented towards chemical biology, molecular pharmacology and drug delivery, the instrument is to performed routine real-time (i.e. immediate access by multiple users) and accurate chromatographic and mass spectrometric analyses of crude synthetic samples, i.e. prior to purification, as well as the exact mass determination of purified samples. The analysis of crude samples is a critical step to ascertain the presence of the desired product prior to purification as well as to identify possible side reactions. In contrast to other liquid chromatography coupled to mass spectrometry instruments available in the Faculty of Chemistry and Pharmacy at the Ludwig-Maximilians-University in Munich, the new instrument should be adapted to large synthetic molecules, i.e. with m/z up to 20 000 Da. Typical samples include large synthetic foldamers specific to new research in the department of Pharmacy, as well as large peptides, small chemically synthesized proteins, foldamer-peptide and foldamer-protein covalent adducts, and complex lipid/polymer/nucleic acid conjugates. While the need for such analyses was only diffuse throughout the faculty of chemistry and pharmacy, punctual need being expected by many research groups, it became acute after the installation of a new Chair at LMU in September 2017. The instrument is thus to be intensely used by this group while being accessible to the entire Faculty.