Studies on the elimination of Campylobacter jejuni from the intestine of chicken.

本项目的目的是通过控制鸡肠道中的肠道菌群来消灭空肠弯曲菌。主要研究结果如下：1.将益生菌中的枯草芽孢杆菌以0.2%或0.5%的浓度添加到养鸡场的普通日粮中，共培养1周。2.在鸡空肠弯曲菌实验性感染中，接种枯草杆菌和蜡样芽孢杆菌均不能清除鸡肠道中的空肠弯曲菌。3.用盲肠内容物口服接种无菌小鼠可建立鸡的肠道菌群。鸡肠道优势菌群双歧杆菌、消化球菌科、类杆菌和梭状芽孢杆菌也是无菌小鼠肠道优势菌群。然而，在接种氯仿处理的鸡盲肠内容物的无菌小鼠中，盲肠内容物的稀释和EG琼脂平板上的菌落悬液厌氧接种鸡盲肠内容物，空肠弯曲菌没有被消灭。5.从鸡粪中分离出的8株肠球菌、肠杆菌科、梭状芽胞杆菌、类杆菌和优生菌经口接种无菌小鼠。在这些灵知生菌小鼠中，空肠弯曲菌并未被消灭。

The purpose of this project is to eliminate C.jejuni by controlling the intestinal flora from the intestine of chicken. Our results were as follows :1.Bacillus subtilis which is one of probiotics was mixed in normal diet at 0.2 or 0.5% for 1 week in a farm of chicken. Consequently, C.jejuni was eliminated from the intestine of chicken.2.In the experimental infection of C.jejuni, inoculation with B.subtilis and B.cereus were unable to eliminate C.jejuni from the intestine of chicken.3.We were able to establish the intestinal flora of chicken in germfree mice by oral inoculation with their cecal contents. The dominant flora in the intestine of chicken, e.g.Bifidobacterium, Peptococaceae, Bacteroidaceae and Clostridium were also dominant flora in the intestine of germfree mice.4.C.jejuni was eliminated after oral inoculation in chicken flora mice. However, in germfree mice inoculated with chloroform-treated chicken cecal contents, 10^<-6> dilution of cecal contents and the suspension of bacterial colonies on EG agar plats inoculated with cecal contents of chicken anaerobically, C.jejuni was not eliminated.5.Eight strains of Enterococcus, Enterobacteriaceae, Clostridium, Bacteroidaceae, and Eubacterium isolated from feces of chicken-flora mice were inoculated orally into germfree mice. In these gnotobiotic mice, C.jejuni was not eliminated.