Histopathological Investigation on the mechanism of generation, proliferation and metastasis of Oral Squamou Cell Carcinoma.

一株新的口腔鳞状癌细胞MISK 81-5的建立及其生物学特性已在日本《癌症研究杂志》上发表。MISK 81-5细胞表现为非整倍体染色体，并向培养基中释放组织蛋白酶D，该蛋白酶被认为与癌细胞的侵袭能力有关。干细胞实验和酶联免疫吸附试验表明，该细胞系还能产生高水平的粒细胞集落刺激因子。通过将人组织蛋白酶D cDNA转染到细胞系中，对其侵袭能力的改变进行了实验研究。针对与细胞表面转化和粘附分子相关的未知抗原的抗体制备也在研究中。用PCNA免疫染色和AgNORs染色观察宫颈和口腔黏膜的增殖活性。这些研究揭示了宫颈和口腔黏膜增生活性与严重程度的密切相关，明确了口腔黏膜严重上皮发育不良的主要组织学组成。这些研究结果发表在《人类病理学》杂志上。目前正在对口腔癌前病变中抑癌基因p53的定位进行免疫组化检测，并利用PCR和原位杂交方法检测其mRNA和点突变。研究了组织蛋白酶D和E在原发性胃癌组织中的免疫组化定位。结果表明，这些蛋白酶在肿瘤细胞的晚期合并蛋白中强烈表达，提示其与肿瘤细胞的局部侵袭和淋巴结转移密切相关。这项研究已提交给一家杂志。

Establishment and biological characteristics of a new oral squmaous carcinoma cell line, MISK 81-5, had been published in Japanese Journal of Cancer Research. MISK 81-5 cells showed an aneuploid chromosomal, and released cathepsin D into culture media, which protease is supposed to correlate with invasive potencies of carcinoma cells. This cell line alos produced a high level of granulocyte colony-stimulating factor demonstrated by stem cell assay and ELISA.An experimental study on the alteration of the invasive potency has been performed by transfecting a human cathepsin D cDNA into the cell line. Preparations of antibodies against unknown antigens correlated with transformation and adhesion molecules on the cell surface has been also investigating.Proliferative activities of the dysplasia and carcinoma in situ in the uterine cervix, and oral mucosa were investigated by means of PCNA immunostaining and AgNORs staining. These studies disclosed the close correlation between the proliferative activity and the severity of the dysplasia in uterine cervix and oral mucosa, main histological components related to severe epithelial dysplasia of the oral micosa were clarified. The results of these investigations have been published in Human Pathology. Immunohistochemical examinations on the localization of p53, as a tumor suppressor gene in oral precancerous lesion, as well as detections of its mRNA and point mutations by using PCR and in situ hybridization methods, are proceeding.Immunohistochemical localizations of cathepsins D and E in primary gastric cancer were investigated. The results that these proteases were strongly expressed in carcinoma cells at the advancing mergin suggesting a closely correlation with the local invasion of carcinoma cells and following metastsis to the lymph nodes. This sytudy has been submitted to a journal.