Histological cna histochenmical studies on the bone metabolism using g confocal laser scanning microscope.

为了将共聚焦激光扫描显微镜（CLSM）应用于骨代谢的研究，进行了骨组织块染色技术的基本方法，以及CLSM与包括三维观察在内的组织形态计量学方法的同步应用。以上结果为CLSM在以下骨代谢研究中的应用奠定了基础：1）大鼠下颌骨软骨和牙槽骨老化变化的组织化学研究; 2）PTH治疗后骨代谢的骨组织形态计量学和组织化学研究; 3）去卵巢大鼠骨小梁减少模式的组织学研究。24 R·25（OH）_2D_3对大鼠实验性肾间质成骨作用的骨组织形态计量学研究。5）大鼠顶骨骨缺损TGF-β成骨活性的组织化学研究。6)硫酸乙酰肝素蛋白多糖在骨组织中定位的免疫组织化学研究7）破骨细胞和骨细胞中CD 44的免疫组织化学定位8）M-CSF给药后OP/OP小鼠股骨中Mac-2的免疫组织化学定位9）大鼠骨组织中CGRP阳性神经纤维的免疫组织化学定位10）RGD肽对破骨细胞形态学影响的组织化学研究。这些组织形态计量学和组织学的结果表明了CLSM的有效性，同时，CLSM的进一步改进使其能够进行三维定量分析。通过使用CLSM，它具有光和电子显微镜的中间分辨率的细胞学分析的水平上的快速发展的可能性也表明。

In order to use confocal laser scanning microscope (CLSM) for the research on bone metabolism, basic methods which entailed the application of bone staining techniques on histological tissue blocks of bone, and concurrent application of CLSM to a histomorphometrical method including three dimensional observation was carried out. The results of the above served as a basis for the application of CLSM to the following research pertaining to bone metabolism.1)Histochemical studies on the aging changes of the rat mandibular chondyle and alveolar bone.2)Bone histomorphometrical and histochemical studies on bone metabolism after PTH administration.3)Histological studies on the decreasing pattern of bone trabecules using ovariectomized rat.4)Bone histomorphometrical studies on the effect of 24R.25(OH)_2D_3 to the experimental renal osteodistorophy of rats.5)Histochemical studies on the bone formation activities of TGF-b using bone defects of rat parietal bone. 6)Immunohistochemical studies of the location f heparan sulfate proteoglycan in skeletal tissues.7)Immunohistochemical location of CD44 in osteoclast and osteocytes.8)Immunohistochemical studies on the location of Mac-2 in the femur of OP/OP mice after administration of M-CSF.9)Immunohistochemical locations of CGRP positive nerve fibers in the rat bone tissues.10)Histochemical studies on the morphological changes of cultures osteoclasts after the administrations of RGD peptide. These histomorphometrical and histological results made clear the usefulness of CLSM.At the same time, the added improvements of CLSM have made three dimensional quantitative analysis. The possibility of rapid development at the level of cytological analysis through use of CLSM which possesses the intermediate resolution of light and electron microscopy is also indicates.