Role of Marek's disease tumor-associated surface antigen (MATSA) in tumor immunity

The purpose of this study is to investigate The role of Marek's disease tumor-associated surfaceantigen (MATSA) in tumor immunity. Various kinds of antigens are expressed on the cell surface ofMarek's disease (MD) lymphoblastoid cell lines，包括：MATSA， chicken fetal antigen, thrombocyte antigen，主要历史研究与其他研究我们examined the biochemical characteristics of MATSA expressed on MSB1-Clo.18 cells using amonoclonal antibody (2b9). The results are summarized as follows:1. The reactivity of 2b9 to MATSAon transplantable MSB1-Clo.18 cells was inhibited by anti-MATSA rabbit serum at a maximum of 855%，与丢失的cells with trypsin or pronase. the reactivity also decreased to about80 % by treatment of the cells with tunicamycin.2. the antigenic activity of MATSA was stable byheating at 56c for 30 minutes, but was unstable by heating at 56c for 30 minuteswas lost by heating at 73 - C for 30 minutes. The activity was stable at neutrality or alkalinity，but was unstable at acidity below pH . 6.0. Further，the activity decreased to 20- 30% by sodium metaperiodate treatment，但它是稳定的，因为neuraminidase treatment. The purified MATSA应该有一个应用程序molecular weight of 70 kilodalton by SDS-PAGE analysis。

The purpose of this study is to investigate the role of Marek's disease tumor-associated surface antigen (MATSA) in tumor immunity. Various kinds of antigens are expressed on the cell surface of Marek's disease (MD) lymphoblastoid cell lines, including MD tumor-associated surface antigen (MATSA), chicken fetal antigen, thrombocyte antigen, major histocompatibility antigen and other antigens. In the present study, we examined the biochemical characteristics of MATSA expressed on MSB1-Clo.18 cells using a monoclonal antibody (2B9). The results are summarized as follows:1. The reactivity of 2B9 to MATSA on transplantable MSB1-Clo.18 cells was inhibited by anti-MATSA rabbit serum at a maximum of 85.5 %, and lost by treatment of the cells with trypsin or pronase. The reactivity also decreased to about 80 % by treatment of the cells with tunicamycin.2. The antigenic activity of MATSA was stable by heating at 37゜C for 30 minutes, but was unstable by heating at 56゜C for 30 minutes, and was lost by heating at 73゜C for 30 minutes. The activity was stable at neutrality or alkalinity, but was unstable at acidity below pH 6.0. Further, the activity decreased to 20-30 % by sodium metaperiodate treatment, but was stable by neuraminidase treatment. The purified MATSA was shown to have an apparent molecular weight of 70 kilodalton by SDS-PAGE analysis.