Experimental Research of Pathophisiology and Treatment of Cerebral Vasospasm in Rabbit Model with Neurologic Symptoms, - The Effect of Elastase Substance Administration -

*症状性血管痉挛实验模型及脑血流、感觉诱发电位（SEP）及病理观察：在两次蛛网膜下腔出血诱导前2周结扎双侧颈总动脉。兔在蛛网膜下腔出血后第4天或第5天表现出严重的神经功能缺损。蛛网膜下腔出血后第4天，症状兔皮质血流量较正常兔减少20-30%。SEP也几乎发生了变化。病理观察中，有症状的家兔基底动脉出现特征性血管痉挛。而脑组织的损伤变化最小。据推测，兔子出现症状是因为它们不再有侧支血流来补偿蛛网膜下腔出血后基底动脉血流的减少。这种症状性血管痉挛模型可能对未来的研究有益。我们正在尝试制造一种症状更严重的新型号。*给药弹性蛋白酶的作用：对于症状性血管痉挛的保护，预期弹性蛋白酶物质的作用是防止弹性层的损伤。在这个系列中，实验了不同数量和方法的弹性物质的管理。结果几乎是否定的。目前正在研究与其他材料的联合处理。

*Experimental model of symptomatic vasospasm and investigations of cerebral blood flow, sensory evoked potetial (SEP) and pathologic findings : The common carotid arteries were ligated bilaterally 2 weeks before twice induction of subarachnoiu hemorrhage in rabbits. Rabbits showed a progressing neurologic deficit that was worst on the fourth or fifth day after the subarachnoid hemorrhage. On the fourth day after the subarachnoid hemorrhage, the cortical blood flow showed 20-30% reduction in symptomatic rabbits in comparison with normal ones. SEP also showed nearly changes. In pathological investigations, Characteristic findings of vasospasm were observed on the basilal artery of symptomatic rabbits. However, the infraction changes of cerbral tissues were minimum.Presumably, the rabbits became symptomatic because they no longer had collateral blood flow to compensate for the reduced blood flow in the basilar artery after subarachnoid hemorrhage. This model of symptomatic vasospasm may be beneficial for future studies. We are now trying to make a new model with more serious symptoms.*The effect of elastase administration : For the protection of symptomatic vasospasm, the effect of elastase substance that prevents the damage of elastic layers was expected. In this seriese, the administration of elastase substances in various amounts and methods were experimented. The results were almost negative. The combined treatment with other materials are investigating now.