Transplantation of the Sympathetic Ganglion in Parkinsonism

在1992-1993年国家科学研究资助计划（C）的资助下，我们对移植的长期培养的交感神经节进行了活性研究。1992年，在6-OHDA损伤的大鼠中检查了长期培养的交感神经节的活力。培养2周或4周的神经节在宿主脑中存活良好，并显示6-OHDA损伤大鼠的异常行为恢复。而培养6周的神经节移植物不能存活，行为不能恢复。1993年，我们在神经节的培养过程中，在培养液中加入白细胞介素-1诱导神经生长因子。在白细胞介素-1条件培养基中培养的交感神经节显示出高浓度的神经生长因子（比对照高7倍）和延长的神经突生长。结果表明，在IL-1条件培养液中培养的交感神经节在宿主脑中的存活率高于对照组，提示在培养系统中处理交感神经节4周可获得高存活率的移植物，该方法具有临床应用价值。

By the support of Grant-in-Aid for Scientific Research (C) from 1992-1993, we have studied viability of transplanted sympathetic ganglion which had been cultured for a long period. In 1992, viability of the long-term cultured sympathetic ganglion was examined in 6-OHDA lesioned rats. The ganglion cultured for 2, or 4 weeks well survived in the host brain and revealed recovery of abnormal behavior of the 6-OHDA lesioned rats. In contrast ganglion cultured for 6 weeks failed to show survival of the graft and recovery of the behavior. In 1993, during culture of the ganglion, we added Interleukin-1 to induce nerve growth factor in the cultured medium. The sympathetic ganglion cultured in the Interleukin-1-conditioned medium showed high concentration of nerve growth factor(seven times higher than the control) and extended neurite outgrowth. Transplantation of the cultured ganglion in the Interleukin-1-conditioned medium revealed higher viability than the control in the host brain.These results indicate the possibility of that the sympathetic ganglion can be treated in the culture system for 4 weeks to obtain high viability of the transplant, and this procedure can be clinically applied in the future.