DIELECTRIC SPECTROGRAM FOR EVALUATION OF ISCHEMIC INJURY OF CIRRHOTIC LIVER

1.在实验性常温肝脏缺血再灌注期测量了肝脏的电阻抗。电阻率(Rho)在缺血后即刻升高，在缺血后约40min达到最大值(Ro_1)，此后缓慢下降。RO2为再灌注前的值。Ro_2/Ro_1比值与再灌流60min后胆汁流量恢复率呈显著正相关。提示Ro_2/Ro_1可作为预测热缺血损伤大鼠肝脏活力的可靠指标。2.胆碱缺乏饮食诱导的脂肪肝缺血再灌注期Rho的变化较正常肝脏轻微。3.在临床肝切除中，正常肝脏的Rho在肝脏缺血50分钟后迅速升高，之后缓慢下降。这些变化与实验中正常大鼠肝脏的变化相同。另一方面，脂肪肝或肝硬化组的Rho变化较正常肝组轻微。在正常大鼠肝脏冷缺血实验中，在生理盐水和UW液中，缺血开始时的%tan Delta与丢失的tan Delta的比率分别在缺血后4h和12h下降。%tan delta值与冷缺血前的%ATP值之间存在显著的相关性。提示在冷缺血后4小时和12小时，冷生理盐水和冷UW液中肝组织的ATP水平均可反映肝组织的ATP值。肝组织中水分含量不影响黄褐斑三角洲的丢失。

1.We measured electrical impedance of the liver in experimental nornmothermic hepatic ischemia-reperfusion period. Resistivity (rho) increased immediately after the initiation of ischemia and showed the maximum value (rho1) about 40 minutes after ischemia and slowly decreased thereafter. rho2 was the value just before the reperfusion. There was significant correlation ship between rho2/rho1 and the recovery rate of bile flow 60 minutes after the reperfusion. This result suggested that rho2/rho1 would be an reliable indices predict the viability of the warm-ischemically injured liver of the rat.2.Changes in rho of the choline deficient diet induced fatty liver during ischemia-reperfusion period was slighter than those of healthy liver.3.In the clinical hepatic resection, rho of the healthy liver showed rapid increased after hepatic ischemia until 50 minutes and slowly decreased thereafter. These changes were the same manner as those of healthy rat liver in the experiment. On the other hand, changes in rho of the fatty liver or cirrhotic liver was slighter than those of healthy liver. These data suggested that slight changes in rho during ischemia should indicate that livers were not healthy.4.In the experiment of cold ischemia of the healthy rat liver, % tan delta, which was the ratio to loss tan delta at the beginning of ischemia, decreased until four hours and 12 hours after ischemia in saline and UW solution, respectively. There was significant correlation ship between % tan delta and % ATP, which is the ratio to the ATP just before cold ischemia. These suggested that loss tan delta would indicate the ATP level of the liver until four hours and 12 hours after cold ischemia, in cold saline and in cold UW solution. Loss tan delta did not affected by the water contents in the liver.