Studies on population genetic informatics and conservation genetics in fish culture and stock enhancement by computer simulation

亲鱼的管理策略不仅是水产养殖种鱼可持续生产的重要内容，也是种群优化计划的重要内容。人工繁殖的放流种鱼必须与野生种群遗传等同。本研究针对野生种群和亲鱼种群遗传分化保护的若干问题，通过微卫星(MS)和mtDNA多态等DNA标记对其进行评估，以获得有关生产合格种鱼和维持适当遗传分化水平的有用信息。利用方正种群的基因数据，通过计算机模拟的平均标记等位基因数和杂合度等指标估计了30代的遗传变化。还提出了DNA标记辅助的亲鱼管理系统，以获得遗传健康的水产养殖鱼种，并进行遗传负责的种群改良活动。

The management strategy of broodstock must be important in the sustainable production of seed fish not only for aquaculture but also for stock enhancement program where the artificially produced seed fish for release is required to be genetic equivalency with the wild population.In this study, several problems related with conservation of genetic divergence in wild population and broodstock were targeted to obtain useful information concerning with production of qualified seed fish and maintaining appropriate level of genetic divergence which were evaluated by the DNA markers such as microsatellite (MS) and mtDNA polymorphis. Using genotype data of founder stock, the genetic changes in 30^<th> generations were estimated by such indicators of the average number of marker alleles and heterozygosity based on computer simulation. The broodstock management system assisted by DNA markers was also proposed to make a genetically healthy fish seed for aquaculture and to perform the genetically responsible stock enhancement activity.