Reserch of Clinical islet transplantation from non-heart beating donor using 2-step two-layer method

2006年3月在胰岛移植工作小组会议上被批准为西近畿和中国地区的胰岛移植中心。我们的胰岛移植计划是这样开始的：我们派遣专门的团队从无心跳的供体那里获取器官，并在运输过程中通过双层方法保存人类胰腺。我们通过Ricordi方法从胰腺分离人胰岛并检查胰岛回收。我们在分离过程后和通过双层方法培养胰岛一天后对人类胰岛进行形态和功能评估。当胰岛回收率超过5000 IE/kg受体体重时，我们对1型糖尿病患者进行胰岛移植，通过动物实验，我们证实并报道了在胰岛分离前应用双层胰腺保存法改善胰岛回收率和功能。此外，我们还研究了两层法用于胰岛细胞培养的有效性， ...更多信息 lture。以上实验表明两步两层法在胰岛移植中的重要性。2005年我们进行了三例人胰岛分离。从三个病例中的一个，我们进行了胰岛移植的受体1型糖尿病。供体为32岁女性，热缺血时间为6 min，冷缺血时间为193 min，双层法保存时间为165 min。纯化前回收率为545267 IEQ，纯化后回收率为483545 IEQ。这符合胰岛移植的标准。接受者是一名30岁的女性，患有不稳定的1型糖尿病。胰岛移植后可检测受体内源性胰岛素的释放。2004年，我们证实了两步两层法的优越性。在2005年，我们展示了从热缺血损伤的胰腺进行胰岛移植的可能性。在临床上，我们报告了一个有价值的情况下，成功的人胰岛移植从一个年轻的，低体重指数，无心脏跳动的尸体。少

Our institute was approved as an islet transplantation center of west Kinki and Chugoku area at a meeting of the islet transplant working group in March 2006. We started our islet transplant program as follows.We send our special team for organ procurement from a non-heart-beating donor and preserve a human pancreas by the two-layer method during transportation. We isolate human islets from the pancreas by the Ricordi method and examine islet recovery. We perform morphological and functional assessments of human islets after the isolation process and after one-day culture of islets by the two-layer method. When the islet recover is over 5000 IE/kg of a recipient weight, we perform islet transplantation to a patient of type 1 diabetes.By animal experiments, we certified and reported improvement of islet recovery and function by applying the two-layer method for pancreas preservation prior to islet isolation. And furthermore we investigated usefulness of the two-layer method for islet cu … More lture. The above experiments show importance of the two-step two-layer method for islet transplantation.In 2005 we performed three cases of human islet isolation. From one of the three cases, we performed islet transplantation to a recipient of type 1 diabetes. Donor was 32 year old female, warm ischemia time was 6min, cold ischemic time was 193min, and preservation period by the two-layer method was 165min. Pre purification recovery was 545267 IEQ, and post purification recovery was 483545 IEQ. This was up to the standard of islet transplantation. The recipient was a 30-year old female of unstable type 1 diabetes. We can detect endogenous insulin release in the recipient after islet transplantation. The recipient was dissolved unstableness of blood glucose level.In 2004, we confirmed superiority of the two-step two-layer method. In 2005, we showed the possibility of islet transplantation from the pancreas damaged by warm ischemia. Clinically we reported a valuable case of successful human islet transplantation from a young, low BMI, non-heart-beating cadaver. Less