Establishment of various measures against infectious diseases between domestic and wildlife animals, preventing the development of stock breeding in southern Africa

1.家畜与野生动物之间的传染病在赞比亚对传统牛进行了BIV血清学监测。BIV pol基因产物与美洲株同源性为98.0 ~ 100%。在跳羚中检测到的DNA序列与伽玛疱疹病毒科的同源性为44-72%，表明未识别的新型病毒。从坦桑尼亚传染性法氏囊病病毒VP2高变区核苷酸序列中，证实了非洲和欧洲/亚洲的变异。抗蜱疫苗的研制利用长角血蜱蛋白酶抑制剂cDNA末端的快速扩增和基于保守分离蛋白设计的引物作为抗蜱疫苗。给牛接种阑尾根头虫seprine抑制剂组合疫苗，可获得对蜱虫的保护性免疫。这是关于抗蜱疫苗试验的第一份报告。新诊断技术的发展种特异性环介导等温扩增（LAMP）方法是一种新型的核酸检测方法。我们将此方法应用于马体浆虫病、牛伊犁菌病和锥虫病的诊断。本研究采用等温和嵌合引物启动核酸扩增两步法对赞比亚产的invA沙门氏菌进行扩增，并用ELISA法对扩增产物进行检测。

1.TRANSMISSIBLE DISEASES BETWEEN DOMESTIC AND WILD ANIMALSSerological surveillance of BIV was conducted in traditional cattle in Zambia. BIV pol gene products showed 98.0-100% homology to the American strain. DNA sequences detected in springboks showed 44-72% homology to the Gammaherpesvirinae, indicating unrecognized novel virus. From nucleotide sequences of the VP2 hypervariable region of infectious bursal disease viruses in Tanzania, both African and European/Asian variants were demonstrated.2.DEVELOPMENT OF ANTI-TICK VACCINERapid amplification of the cDNA ends and primers designed based on a conserved seprin were used for the protease inhibitor from Haemaphysalis longicornis as an anti-tick vaccine. Vaccination of cattle with a combination of Rhipivcephalus appendiculutus seprine inhibitor conferred protective immunity against ticks. This is the first report on an anti-tick vaccine trial.3.DEVELOPMENT OF NEW DIAGNOSTIC TECHNOLOGYSpecies-specific loop-mediated isothermal amplification (LAMP) method is a novel nucleic acid method. We used this method for the diagnosis of equine piroplasmosis and bovine theileriosis and trypanosomosis. We tried a 2-step method using isothermal and chimeric primer-initiated amplification of nucleic acids to amplify the Salmonella invA and detecting the amplified product using ELISA from carcass samples in Zambia.