Molecular mechanisms of gametophytic self-incompatibility in Prunus species

李树是蔷薇科的一个属，包括重要的商品果树和坚果树。大多数李属植物表现出单因子配子体自交不亲和系统，该系统由单个位点s位点控制，具有多个等位基因。由于李属植物不能单性结果，除非与具有不同s基因型的品种授粉，否则它们不会结果。因此，确定正确的花粉不亲和性类群并将品种分配到这些类群中是保证作物高产的必要条件。本研究鉴定了甜樱桃、杏仁、日本李和日本杏中与自交不亲和相关的S-RNases。编码s - rnase的cdna已被克隆并进行了dna测序，从而建立了这些水果s -等位基因的分子分型系统。此外，还发现了可作为日本杏自交亲和分子标记的S-RNase等位基因。

Prunus, one of the genera in the Rosaceae, includes important commercial fruit and nut tress species. Most of the Prunus species exhibit the monofactorial gametophytic self-incompatibility system which is controlled by a single locus, the S-locus, with multiple alleles. As Prunus species are unable to bear fruit parthenocarpically, they will not set fruit unless pollinated with cultivars with different S-genotypes. Thus, determination of correct pollen incompatibility groups and assignment of cultivars to the groups are essential for good crop production.This study identifies S-RNases associated with self-incompatibility in sweet cherry, almond, Japanese plum, and Japanese apricot. cDNAs encoding the S-RNases have been cloned and DNA-sequenced, which leads to the development of a molecular typing system for S-alleles in these fruit species. Furthermore, S-RNase allele that can be used as a molecular marker for self-compatibility in Japanese apricot has been explored.