Molecular epidemiology of environmental and hereditary factors in esophageal canser

在中国食管癌高发区，对患者组(79人)和居民(499人)进行了营养方式调查。选择性别和年龄(±1)相匹配的对照组(男性42例，女性30例)，明确食道癌的危险因素。食道癌家族史、劳动强度大、使用井水频率明显高于对照组，冬季蔬菜摄入量低于对照组。患者组猪肉脂肪和玉米的摄入量也高于对照组，而大豆、土豆、海藻和水果的摄入量低于对照组。由于食道癌高发区居民大豆、土豆、海藻、水果的摄入量明显低于低发区居民，说明食道癌患者这些食物的摄入量相当低，而这些L…对中国地区77例食管癌高发区患者和50例低发区食管癌患者的肿瘤组织中P53基因突变谱进行了研究。P53基因突变的患者频率约为50%，两个地区之间的频率没有差异。但突变谱略有不同：高发区患者的突变主要发生在外显子5和8(72.2%)，而低发区患者的突变主要发生在外显子7。比较有无P53基因突变患者的营养状况。P53基因突变患者中，P53基因第7、8外显子突变占80%，且有愿意吃辣、善用麻辣食物的习惯。所有P53基因突变患者和使用井水的患者均使用原水。分析同一肿瘤组织中p16、p15、E-钙粘蛋白、H-钙粘蛋白和雌激素受体的DNA甲基化情况。DNA甲基化频率在高发区高于低发区。DNA甲基化与营养习惯无相关性。而DNA甲基化主要见于父亲有食道癌家族史的患者。较少

In a high-incidence area of esophageal cancer in China, the survey of nutritional styles was carried out for the patient group(79 persons)and inhabitants(499 persons). The sex- and age(±1)matched controls(M, 42 and F, 30)were selected, and the risk factors of esophageal cancer were clarified. The frequencies of family history of esophageal cancer, heavy work, use of well water were significantly higher in the patient group than in controls, whereas those of intakes of vegetables in the winter were lower in the former. The intakes of pork fat and corn also higher in the patients group than in controls, whereas those of soybeans, potatoes, seaweed, and fruit were lower in the former group. Since the intake of soybeans, potatoes, seaweed, and fruit were significantly lower in the inhabitants living in the-high incidence area of esophageal cancer than those in the low-incidence area, suggesting that the intakes of these foods in esophageal cancer patients were considerably low, and these l … More owered intakes seem to be risk factors of esophageal cancer.P53 mutation spectrum was investigated in tumor tissues from 77 esophageal cancer patinets living in the high-incidence area and 50 patients living in the low-incidence area in China. The frequencies of patients with p53 mutations were about 50%, and no difference in the frequencies was seen between both area. However, the mutationl spectrum was slightly difference : the mutations were mainly observed in exonss 5 and 8(72.2%)ih patients from the high-incidence area, whereas, those were in exons 7 in the patients from the low-incidence area. The nutritional styles of the patients were compared between the patients with and without p53 mutations. The patients with p53 mutation engaged in a light work, , and had a habit which ate the spicy food willingly and use of well ; of the patients having a habit of spicy food, 80% were mutated on exons of 7 and 8 of p53. Allthe patients with p53 mutation and use of well water used raw water. The DNA methylation of p16, p15, E-cadherin H-cadherin and estrogen receptor was analyzed using the same tumor tissues. The frequencies of the DNA methylation tended to be greater in the high-incidence area than in the low-incidence area. There wsa no corelationship between DNA methylation and nutritional habits. However, DNA methylation was mainly observed in the patients with the family history of esophageal cancer in the father. Less