Development of Strategy for Fermentative Production Based on Symbiosis of Microorganisms

在L. kefiranofaciens和S. cerevisiae的批量混合培养中，可以对L. kefiranofaciens进行对称乳酸、细胞生长和kefiran生产率明显增加，与那些在纯文化中的人进行比较。Kefiran的生产率为36毫克·l^<-1> ·h^<-1>在非有机条件下混合的培养率高于纯培养（24毫克·l^<-1> ·h^<-1>）。Kefiran生产是由联邦批次混合文化中的新介质的补充而进一步增强的。在联邦批次混合的文化中，最后一次以5.41 g ·l^<-1>完成于87小时。Simulation study considered the reduction of lactic acid in pure culture建议，l. kefiranofaciens和s. cerevisiae不仅减少了lactic acid concentration by consumption但也刺激了细胞生长和kefiran production of l. kefiranofaciens。R eduction of hydrogen peroxide did not concern in stimulation of kefiran production of L. kefiranofaciens in mixed culture。它还发现了一个额外的年份的蔓延介质并没有对胶囊kefiran在混合文化中的作用增加的主要影响。在一种非接触的混合文化中，在混合文化中发现的L. Kefiranofaciens和II的胶囊基菲兰生产中也没有增加。在不活性S.的混合文化中增加胶囊kefiran，并同意在模拟生物和气动混合文化中加入良好。在玻璃珠破坏中不活跃的S. Cerevisiae的混合文化中，胶囊Kefiran的增加少于热治疗中不活跃的S. Cerevisiae的混合文化。从这些营养学结果中，它被合并为与S. Cerevisiae增强了L. kefiranofaciens在混合文化中的Capsuular kefiran生产。

In a batch mixed culture of L.kefiranofaciens and S.cerevisiae, which could assimilate lactic acid, cell growth and kefiran production rates of L.kefiranofaciens significantly increased, compared with those in pure cultures. The kefiran production rate was 36 mg ・l^<-1> ・h^<-1> in the mixed culture under the anaerobic condition, which was greater than that in the pure culture (24 mg ・l^<-1> ・h^<-1>). Kefiran production was further enhanced by an addition of fresh medium in the fed-batch mixed culture. In the fed-batch mixed culture, a final kefiran concentration of 5.41 g ・l^<-1> was achieved at 87 h. Simulation study considered the reduction of lactic acid in pure culture suggested that coculture of L.kefiranofaciens and S.cerevisiae not only reduced the lactic acid concentration by consumption but also stimulated cell growth and kefiran production of L.kefiranofaciens. R eduction of hydrogen peroxide did not concern in stimulation of kefiran production of L.kefiranofaciens in mixed culture. It was also found that the addition of spent medium of yeast was not the main effect for increasing in capsular kefiran in the mixed culture. And in a non-contact mixed culture, there was also no increase in capsular kefiran production of L.kefiranofaciens as much as ii was found in mixed culture. Increase in capsular kefiran in the mixed culture with inactivated S.cerevisiae agreed well to that in anaerobic and aerobic mixed culture. In the mixed culture with inactivated S.cerevisiae by glass beads disruption, the increase of capsular kefiran was less than that in mixed culture with inactivated S.cerevisiae by heat treatment. From these fermentation results, it was concluded that the physical contact interaction with S.cerevisiae enhanced the capsular kefiran production of L.kefiranofaciens in mixed culture.