Capillary Sequencer

高分辨率毛细管电泳是精确鉴定DNA多态性的标准方法。毛细管测序仪的主要应用是基因座特异性DNA标记的高通量基因分型，例如不同植物和动物物种中高度可变的核微卫星标记。该仪器还将用于正在进行的和拟议的项目，用于对各种不同生物群中的特定DNA条形码区域进行测序。高精度的基因分型对于群体遗传学和法医学应用，如基因流和亲子关系分析以及遗传变异的评估是必不可少的。这一方法今后还将作为标准方法，在保护方案框架内监测遗传资源和人类对生物多样性的影响。在代表性遗传标记上进行更大样本量的分析对于在知名科学期刊上发表论文变得更加重要。新的仪器将允许在多重反应中分析更大的样本量（每周几千个样本），正如正在进行的和拟议的项目所预期的那样。由于广泛的基因分型方法（例如，通过对微卫星、单核苷酸多态性、全基因组AFLP）和DNA条形码区域测序的研究，该仪器将被不同院系（林业、生物学）的不同研究小组使用，确保系统的持续利用。该仪器将成为一个服务单元的一部分，该服务单元还包括一个质谱仪，用于大规模评估单核苷酸多态性（SNP）和表观遗传变异。

High-resolution capillary electrophoresis is a standard method for precise identification of DNA polymorphisms. The main application of the capillary sequencer is the high-throughput genotyping of locus-specific DNA markers such as highly variable nuclear microsatellite markers in different plant and animal species. The instrument will also be used in ongoing and proposed projects for the sequencing of specific DNA-barcoding regions in a variety of different organismal groups. High-precision genotyping is indispensable for population genetic and forensic application such as gene flow and parentage analyses and for the assessment of genetic variation. This method will be used also in the future as standard method for the monitoring of genetic resources and of human impacts on biological diversity in the framework of conservation programs. The analysis of larger sample sizes at representative genetic markers is becoming even more important for the publication in renowned scientific journals. The new instrument would allow the analysis of larger sample sizes in multiplex reactions (several thousand samples per week) as anticipated in ongoing and proposed projects. Because of the wide spectrum of genotyping methods (e.g., microsatellites, SNPs, genome-wide AFLPs) and the sequencing of DNA-barcoding regions, the instrument would be used by different research groups of different faculties (Forestry, Biology), securing a continuous utilization of the system. The instrument would become part of a service unit which also includes a Mass spectrometer for the large-scale assessment of Single Nucleotide Polymorphisms (SNPs) and of epigenetic variation.