Allograft Rejection in FcR γ knockout mice

为了阐明Fc受体在同种异体排斥反应过程中的作用，对Fcrγ基因敲除小鼠进行了同种异体移植。皮肤移植是在MHC血型不合的小鼠(BALB/c→C57BL/6)之间进行的。受体C57BL/6小鼠为FcRγ缺陷小鼠或野生型对照小鼠。Fcrγ基因敲除小鼠和对照组的同种异体皮肤移植在移植后7天或8天内均出现排斥反应，移植皮肤存活时间在两组之间没有差异。接下来，为了研究细胞表面表达Fc受体的巨噬细胞在同种异体移植排斥反应中的作用，在MHC血型不合的大鼠组合中进行了异位气管移植。受体大鼠在第0、7、14天注射具有清除巨噬细胞能力的GdCl3，第21天收获同种异体气管。GdCl3处理的动物与对照组相比，阻塞性呼吸道疾病的严重程度显著降低，这种疾病与同种异体移植排斥反应相关。提示巨噬细胞在大鼠异位气管移植排斥反应和阻塞性呼吸道疾病的发生发展过程中起重要作用。

To clarify the role of Fc receptors in the process of allograft rejection, allotransplantations were performed in FcR γ knockout mice. Skin transplantation was performed between a MHC incompatible combination of mouse (BALB/c→C57BL/6). Recipient C57BL/6 mice were those deficient in FcR γ or wild-type controls. Skin allografts in FcR γ knockout mice and the controls were both rejected within day 7 or 8 post-transplant and there was no difference in the duration of graft survival between the groups. Next, to investigate the role of macrophages that express Fc receptors on cell surface in allograft rejection, heteropic tracheal transplantation was performed in a MHC incompatible combination of rat. Recipient rats were injected with GdCl3, which has an ability to deplete macrophages, on day 0, 7, 14 and tracheal allografts were harvested on day 21. The severity of obstructive airway disease, which is known to develop in association with allograft rejection, was significantly reduced in GdCl3-treated animals compared to controls. This indicates that macrophages play a role in the process of allograft rejection and the development of obstructive airway disease in a model of heterotopic tracheal transplantation in rats.