Periodontal tissue regeneration with autologous cell transplantation of bone marrow mesenchymal stem cells in dogs

一些研究表明，间充质干细胞（MSC）可以分化为各种结缔组织细胞，可用于自体细胞移植。在这项研究中，我们研究了MSC移植到牙周缺损的有用性。从比格犬中分离骨髓MSC并在体外扩增。将扩增的MSC与不同浓度（0、2 × 10^6、5 × 10^6、1 × 10^7、2 × 10^7个细胞/ml）的2%端肽胶原混合，并自体移植到实验性III类缺损处。在移植后一个月对牙周组织愈合进行组织学和形态计量学分析。2 × 10^7细胞/ml组牙槽骨、牙周膜和牙骨质几乎完全再生。形态计量学分析显示，2 × 10^7个细胞/ml组的新骨和牙骨质形成率显著高于0个细胞/ml组（p<0.01）。这些结果表明，自体骨髓间充质干细胞移植可能成为牙周组织再生的一个有用的选择。

Some studies have shown that mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells are useful for autologous cell transplantation. In this study, we examined usefulness for MSC transplantation into the periodontal defect. Bone marrow MSC were isolated from beagle dogs and expanded in vitro. The expanded MSC were mixed with 2% atelocollagen in various concentration (0, 2x10^6, 5x10^6, 1x10^7, 2x10^7 cells/ml) and auto-transplanted to experimental class III defects. Periodontal tissue healing was evaluated both histological and morphometrical analyses at one month after transplantation. The defects were almost completely regenerated with alveolar bone, periodontal ligament and cementum in 2x10^7 cells/ml group. Morphometrical analysis revealed that newly formed bone and cementum rates in the 2x10^7 cells/ml group was significantly higher than that in 0 cells/ml group (p<0.01). These results indicate that the auto-transplantation of bone marrow MSC might become a useful option for periodontal tissue regeneration.