Liquid chromatography-mass spectrometry system (LC-MS)

对于生物学和生物化学的许多领域，高分辨率质谱学已经成为检查细胞和组织组成的宝贵工具。在这项提案中，我们申请了一个高分辨率的液体色谱质谱(LC-MS)系统，该系统将被集成到莱比锡大学生命科学学院的质谱学核心设施中。虽然这一核心设施运行着专门分析蛋白质和多肽的仪器，但所要求的LC-MS系统将允许发现和量化复杂细胞提取物中的代谢物。几个研究小组在一些项目中需要新仪器，包括分析微藻的次生代谢物，调查化学多样性在陆地植物-草食动物相互作用中的作用，或可靠地检测复杂混合物中的痕量分析物(例如废水中的除草剂)。此外，所要求的仪器需要更大的质量范围(m/z 50到6000)，以表征分子量高达150 kDa的大的完整蛋白质。新的LC-MS系统将加速莱比锡大学生命科学学院现有的研究项目，并促进建立新的合作和研究联盟。

For many areas of biology and biochemistry, high-resolution mass spectrometry has become an invaluable tool to examine the composition of cells and tissues. In this proposal, we apply for a high-resolution liquid-chromatography mass spectrometry (LC-MS) system, which will be integrated into the Mass Spectrometry Core Facility of the University of Leipzig's Faculty of Life Sciences. While this core facility runs instruments specialised in the analysis of proteins and peptides, the requested LC-MS system will allow the discovery and quantification of metabolites in complex cellular extracts. The new instrument is required by several research groups for a number of projects, including the analysis of secondary metabolites from microalgae, the investigation of the role of chemodiversity role in land plant-herbivore interactions, or the reliable detection of trace amounts of analytes in complex mixtures (e.g. herbicides in waste water). In addition, the requested instrument requires an extended mass range (m/z 50 to 6000) in order to characterise large intact proteins with a molecular weight of up to 150 kDa. The new LC-MS system will accelerate existing research projects at the University of Leipzig's Faculty of Life Sciences and facilitate the establishment of new collaborations and research consortia.