Gene analysis and in vitro culture of the new human malaria parasites

利用从缅甸和印度尼西亚采集的三日疟原虫和卵形疟原虫新类型的野生分离株，分析了SSUrRNA、细胞色素B、半胱氨酸蛋白酶或动合子表面抗原基因。此外，使用野生分离株检查体外培养。（1）新类型卵圆毕赤酵母SSUrRNA基因有3个不同的序列，均与经典类型卵圆毕赤酵母的两个序列不同。（2）三日疟原虫和卵形疟原虫新类型的半胱氨酸蛋白酶基因序列与各经典类型乌干达1型和乌干达1型的半胱氨酸蛋白酶基因序列不同。（3）新卵圆线虫的动合子表面抗原基因序列与新卵圆线虫Ⅰ型不同。(4)In细胞色素B基因序列分析表明，新的卵圆假单胞菌与Harding株的细胞色素b基因序列不同，而新的三日假单胞菌与Uganda-1株的细胞色素b基因序列也不同，但两种新类型之间没有差异。(5)In使用含有葡萄糖和20%人血清的富集培养基进行这些新的人疟疾寄生虫的体外培养。在培养24-48小时后，它们生长到幼鞭毛期，但未观察到进一步生长。

Using wild isolates of the new types of P. malariae and P. ovale collected from Myanmar and Indonesia, SSUrRNA, cytochrome b, cysteine protease or ookinete surface antigen genes were analyzed. In addition, in vitro culture was examined using wild isolates.(1)The new type of P. ovale possessed three different sequences of SSUrRNA gene, and all of them were differed from two sequences of the classic type, Nigerian-I.(2)The new types of P malariae and P. ovale had different sequences of cysteine protease gene from each classic type, Uganda-1 or Nigerian-I.(3)The new P ovale had different sequences of ookinete surface antigen genes from Nigerian-I.(4)In analysis of cytochrome b gene, the new P. ovale had a different sequence from the Harding strain, whereas the new P. malariae were also different from Uganda-1, but there was no difference between the two new types.(5)In vitro cultures of these new human malaria parasites were performed, using an enriched medium with glucose and 20% human serum. After cultivation for 24-48 hrs, they grew to young schizont stages, but further growth was not observed.