The colonizing of periodontopathic bacteria and the transition of inflammation with dental calculus formation periodontal rat model.

大鼠接种牙龈卟啉单胞菌(P.G.)2周(每周3次)。在牙结石形成之后，这被认为是牙周病杆菌。接种前，用磺胺甲恶唑/甲氧苄啶对特定病原菌进行减毒，清除口腔病原菌。在抗菌药物的作用下，P.G.接种了疫苗。给大鼠灌胃Bringer等(1971)处方的牙结石形成食物4周。将大鼠分为4组，1组为阴性对照组(未接种细菌)，其余3组分别改变饲养周期。P.G.在添加血液琼脂(哥伦比亚中央通讯社含5.0μg/ml氯化高铁血红素、0.5μg/ml甲萘二酮和5%去纤维羊血)上培养。在实验中，细菌在添加的血液琼脂上生长，在37℃的厌氧室中，在10%二氧化碳，10%氢气和80%氮气的气氛中生长。将活菌悬液接种于含5%羧甲基纤维素的PBS溶液中。将细菌直接接种于大鼠口腔内。用Bringer等(1971)的方法评价牙石的粘连程度，并评价牙槽骨缺损处的吸收程度。虽然结石在下颌侧的粘连较多，但Pg菌的沉降量呈逐渐减少的趋势。此外，在牙槽骨吸收程度接种P.G.细菌的情况下，结石的粘连程度呈大趋势，与低无关。

Rats were inoculated 2 weeks (three times per week) with Porphyromonas gingivalis (P.g.) that is thought as the periodontopathic bacteia, after dental calculus had been formed. Before inoculation, the specific pathogen were reduced with sulfamethoxazole/trimethoprim and were eliminated oral pathogenic bacteria. After the medicament of anti-bacterial drugs, P.g. was inoculated. The dental calculus formation food by the prescriptions of Bringer et al (1971) was given to rats for 4 weeks. Rats were divided into 4 groups, 1 group was negative control (bacteria was not inoculated), 3 remaining groups were changed breed periods. P.g. was cultivated on supplemented blood agar (Colombia CNA based with 5.0μg/ml hemin, 0.5μg/ml menadione and 5% defibrinated sheep's blood). For experiments, bacteria were grown on supplemented blood agar at 37℃ in an anaerobic chamber under an atmosphere of 10% carbon dioxide, 10% hydrogen and 80% nitrogen. The rats were inoculated live P.g.suspension in 5% carboxymethyl-cellulose in PBS solutions. The bacteria were inoculated directly in the oral cavity of rats. It was estimated the adhesion of calculus with the method of Bringer et al(1971), and evaluated the absorption degree of an alveolar bone defects. Although the adhesion of the calculus is adhering a lot on the side of the lower jaw as a result, the settlement of the P.g.bacteria was in a decrease trend gradually. Also, the adhesion degree of the calculus was in a big trend, without being related to low, in the case that the absorption degree of the alveolar bone inoculated the P.g.bacteria.