Effects of the combined exercise, diet and antihypertensive drug therapy on diabetic nephropaty in rats

本研究旨在观察运动、饮食和ACE抑制剂（captopuril）联合治疗对OLETF大鼠肾功能、BE葡萄糖耐受（GI）和糖尿病（DM）相关血脂异常的影响。将35只雄性大鼠随机分为运动组、饮食组、药物组和联合治疗组（Med&Ex、Ex &Diet）。对21至30周龄的大鼠进行每种处理。在治疗前和治疗后进行口服GI试验。每3周观察一次体重、血压和尿白蛋白排泄率。每次给药后，在麻醉下使用静脉注射法对动物实施安乐死。测量肾脏、心脏和部分肌肉重量，观察肾脏结构变化、血脂和股四头肌酶活性。结果：Ex和Med &Ex治疗抑制了BW的增加，改善了GI。用ACE抑制剂使OLETF大鼠血压降至正常对照LET（）大鼠水平。OLETF-Sed和-Med&Ex组中UAE的增加受到抑制。Ex治疗改善了GI和血脂异常，并增加了四头肌的肌肉质量和酶活性。然而，由于运动时血压升高，并不能充分抑制DN的发展。药物治疗抑制了UAE的增加，但BW增加，GI和血脂异常恶化至与OLETFSed组相同的水平。Med&Ex疗法最大限度地减少了运动的缺点。结论：运动时血压升高是糖尿病大鼠发生糖尿病肾病的危险因素。饮食疗法增强了体育锻炼的优势，同时预防了糖尿病肾病的发生。

The present study was conducted to examine the effects of combination therapy, consisting of exercise, diet and ACE-inbibitor(captopuril), on renal function, BE glucose intolerance(GI) and dyslipidemia associated with diabetes mellitus (DM) in OLETF rats. Thirty-five male rats were randomly assigned to exercise, diet, medication, and combined therapy (Med&Ex, Ex &Diet) groups. Each treatment was conducted on rats 21 td 30 weeks in age. An oral GI test was carried out pre-and post-therapy. Body weight (BW), BP and urine albumin excretion rate (UAE) were observed every three weeks during each treatment. After each treatment, the animals were euthanized using venesection under anesthesia. Kidney, heart and several muscle masses were weighed, and structural changes of the kidney, serum lipids and enzymes activities in quadriceps were examined. Results: The Ex and Med &Ex therapy inhibited increases in BW and improved GI. BP in OLETF rats was decreased to the same level as normal control LET() rats by taking an ACE-inhibitor. Increase in UAE was inhibited in the OLETF-Sed and -Med&Ex groups. Ex therapy improved GI and dyslipidemia, and increased muscle mass and enzymes activities in quadriceps. However, it didn't inhibit the development of DN sufficiently, due to the accompanying increase in BP during exercise. Med therapy inhibited increase in UAE, however, BW was increased and GI and dyslipidemia deteriorated to the same levels as those of the OLETFSed grouj. Med&Ex therapy minimized the disadvantage of exercise. Conclusion: This study demonstrated that elevation of BP during exercise in DM rats was a risk factor for the development of DN. The Med&Ex, diet therapy enhanced the advantage of physical exercise and simultaneously prevented the development of DN.