Molecular genetic and genomic analysis of transcriptional response to high light stress

利用芯片分析了植物对强光、干旱和活性氧的响应，并鉴定了受强光调控的基因。我准备了拟南芥的启动子数据库，并试图利用它来鉴定高光特异性基因激活的顺式调控元件。首先，与整个拟南芥启动子相比，我提取了高光激活启动子组中代表性过高的元件。在转基因拟南芥中，在与荧光素酶报告基因融合后产生强烈的高光响应的ELIP2启动子中发现了这些顺式候选基因。结合其他一些分析，我可以确定两个假定的高光响应元素。一个转基因拟南芥株系通过激活荧光素酶活性来显示对光胁迫的转录反应，用于筛选具有改变的高光反应的突变体。对几个分离的突变体进行了生理分析，以便对突变体进行分类。这些分析表明，突变体有两种类型，一种是信号转导紊乱的突变体，另一种是光合作用紊乱的突变体。

Response to high light, drought, and reactive oxygen was analyzed using microarray, and genes under specific regulation by high light were identified. I have prepared promoter database of Arabidopsis, and with the aid of it, I tried to identify cis regulatory elements for high light-specific gene activation. Firstly, I extracted overrepresented elements among the high light-activated promoter group compared with whole Arabidopsis promoters. Some of these cis candidates were found in the ELIP2 promoter, which gives strong high light response when fused to the luciferase reporter in transgenic Arabidopsis. Together with some other analyses, i could identify two putative high light responsive elements.A line of transgenic Arabidopsis that shows transcriptional response to light stress as activation of luciferase activity was used for screening mutants with altered high light response. Several isolated mutants were subjected to physiological analyses in order to classification of the mutants. These analyses revealed that there are two types of mutants, mutants with the disturbed signal transduction and ones with disordered photosynthetic apparatus.