Ultrasound therapy for soft tissue sarcoma with new quinolone antibiotics as a sonodynamic compound

采用0.2 mM盐酸洛美沙星(LFLX)、司帕沙星(SPFX)、环丙沙星(CPFX)和水合加替沙星(GFLX)作为体外抗肿瘤药物，观察超声(US)对新型喹诺酮类抗生素(NQ)是否具有协同抗肿瘤作用。经2W/cm2US照射30s和60s后，NQ抗生素组肿瘤细胞存活率明显低于未加NQ抗生素组(P&lt；0.001)。在5月-Giemsa涂片中，对照组大部分肿瘤细胞保持完好。而加入0.2 mM SPFX组，肿瘤细胞多呈碎裂状。SPFX的协同抗肿瘤作用也呈剂量依赖关系。此外，D-甘露醇联合SPFX照射后肿瘤细胞存活率与单用SPFX相当，但与L组氨酸合用时，肿瘤细胞存活率显著高于单用SPFX。这些结果表明，NQ抗生素可能是在US辐射下具有抗肿瘤活性的有用化合物，单线态氧的产生参与了细胞内损伤过程。

To determine if there is any synergistic antitumor effect of ultrasound (US) in the presence of new quinolone (NQ) antibiotics, 0.2 mM solution of lomefloxacin hydrochloride (LFLX), sparfloxacin (SPFX), ciprofloxacin hydrochloride (CPFX), and gatifloxacin hydrate (GFLX) were used as sonodynamic compound against sarcoma 180 cells in vitro. After US irradiation at 2 W/cm^2 for 30 and 60 seconds, the survival rates of tumor cells in NQ antibiotics added groups were significantly lower than those without (P<0.001). In May-Giemsa smear, most of tumor cells were maintained intact in control group. However, in 0.2 mM SPFX added group, the tumor cells were mostly broken into pieces. The synergistic antitumor effects of SPFX were also dose-dependent. Furthermore, when D-mannitol was used with SPFX, the survival rate of tumor cells after irradiation was comparable with that when SPFX alone was applied, but when L-histidine was used concurrently, the survival rate of tumor cells was significantly higher than that when SPFX alone was applied. These findings suggest that NQ antibiotics would be useful compounds for antitumor activities under US irradiation, and generation of singlet oxygen was involved in the in cell damage process.