The analysis of the graft from the viewpoint with the relation between malignancy of oral carcinoma and defensin

目的：确定人-β-防御素-2和人α-防御素在牙源性角化囊肿和根性囊肿中的定位。人β-防御素-2免疫反应明显见于牙源性角化囊肿(72%)和根性囊肿(37%)。在两种类型的包囊的角化旁和颗粒层的角质形成细胞的胞浆中都检测到了人β-防御素-2的信号。人β-防御素-2在囊肿和正常粘膜中的定位没有差异。人α-防御素免疫反应在两种类型的包囊中均可检测到，但在角质形成细胞中未检测到，在固有层中性粒细胞的胞浆中可检测到人α-防御素的信号。许多包囊产生人类β-防御素-2和人类芦荟防御素，这表明利用防御素的类似防御机制存在于这两种类型的包囊中，以及正常口腔粘膜中。下一项研究旨在证明具有抗菌活性的人β-防御素在干燥综合征和口干症组织中的免疫组织化学定位和分布。组织标本石蜡包埋，β-防御素采用链霉亲和素-生物素偶联过氧化物酶免疫组织化学染色。对口干症患者和对照组(非口干症和非干燥综合征)的唇腺进行了抗β-防御素抗体染色。然而，干燥综合征患者的唇腺未见抗β-防御素抗体染色，因此，β-防御素被认为是干燥综合征的重要因素。

To identify the location of human-beta-defensin-2 and human alpha-defensins in odontogenic keratocysts and radicular cysts. Human beta-defensin-2 immunoreactivity was evident in odontogenic keratocysts(72%), and in radicular cysts(37%). Signals for human beta-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localization of human beta-defensin-2 did not differ between cysts and normal mucosa. Human alpha-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes, Signals for human aloha-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Many cysts generate human beta-defensin-2 and human aloha-defensins suggesting that similar defense mechanisms utilizing defensins exist in both types of cysts, as well as in normal oral mucosa.Next study was to demonstrate the immunohistochemical localization and distribution of human beta-defensins, peptides with antimicrobial activity, in Sjogren's syndrome and Xerostomia tissues. Tissue samples were embedded in paraffin and beta-defensins were immunostained by the streptavidin-biotin coupled peroxidase method. Labial glands in Xerostomia patients and control patients(non- Xerostomia and non- Sjogren's syndrome) were stained with anti-beta-defensins antibody. However, labial glands in Sjogren's syndrome were not stained with anti-beta-defensins antibody.Therefore, beta-defensins have been suggested to be important factors in Sjogren's syndrome.