The roll of soluble interleukin-1 receptor type 2 for periodontal tissue

首席调查员和调查员提供了这项赠款项目的结果。（1）收集52例牙周炎患者181份龈沟液，采用酶联免疫吸附法（ELISA）检测IL-1 β和可溶性IL-1受体2（sIL-1 Rt 2）。IL-1β、sIL-1 t2水平与牙周病严重程度呈正相关。同时检测了慢性牙周炎（CP）和侵袭性牙周炎（AgP）患者龈沟液中IL-1b和sIL-1 t2的含量。IL-1β在CP组（82.2±12.3ng/ml）和AgP组（68.3±9.4ng/ml）无显著性差异，而sIL-1 Rt 2在AgP组（3.0±0.6ng/ml）明显低于CP组（1.1±0.3ng/ml）。因此，这些结果表明，AgP疾病的特点，快速牙周组织破坏可能与减少sIL-1 Rt 2的生产。(2)A在Depa共招募了102名日本患者和88名牙周健康志愿者 ...更多信息 2002年至2004年在爱知学院大学进行牙周病学研究。直接测序法鉴定多态性。单核苷酸多态性（SNPs）鉴定后，进行等位基因和单倍型分析。在24个AgP和24个对照中，对包含IL 1 R1和IL 1 R2的所有外显子和相关外显子-内含子边界的14.5-kb基因组区域进行测序。在包含IL 1 R1和IL 1 R2的所有外显子和相关外显子-内含子边界的基因组区域内，在48名受试者中鉴定出38个SNP。5个位于编码元件内，2个位于5'侧翼区，22个位于内含子内，9个位于3'非翻译区。IL 1 R2基因3个单倍型和3个SNP的频率在AgP组和对照组之间有显著性差异（p<0.05）。（3）分离慢性牙周炎患者牙龈成纤维细胞。用与AgP密切相关的伴放线放线杆菌脂多糖刺激细胞。检测IL-1β和IL-1 Rt 2 mRNA表达。然而，在培养上清液中仅测量IL-1β的蛋白质产生，而不测量sIL-1 Rt 2。项目目前正在进行不同的实验设计，使用免疫活性细胞，如中性粒细胞或单核细胞。少

Head investigator and investigators have provided the results from this grant projects.(1)One hundred and eighty one gingival crevicular fluids samples were obtained from 52 periodontitis patients and interleukin-1 (IL-1)β and soluble IL-1 receptor type 2 (sIL-1Rt2) were measured by using ELISA assay kits. The concentration of IL-1β and sIL-1t2 were positive correlated with the periodontal disease severity. On the other hand, GCF IL-1b and sIL-1t2 concentrations from chronic periodontitis (CP) patients and aggressive periodontitis (AgP) patients were also examined. IL-1β was no different between CP (82.2±12.3ng/ml)and AgP(68.3±9.4ng/ml), however, AgP sIL-1Rt2(3.0±0.6ng/ml) was significantly decreased compared with CP sIL-1Rt2(1.1±0.3ng/ml). Therefore, these results suggest that AgP disease character of rapid periodontal tissue breakdown may relate to the decreased sIL-1Rt2 production. (2)A total of 102 Japanese patients and 88 periodontally healthy volunteers were recruited at the Depa … More rtment of Periodontology in Aichi-gakuin University from 2002 to 2004. Polymorphisms were identified by direct sequence method. After the single nucleotide polymorphisms (SNPs) identification, allelo- and haplotype analysis were performed. A 14.5-kb genomic region containing all exons and relevant exon-intron boundaries of IL1R1 and IL1R2 were sequenced in 24 AgPs and 24 controls. Within genomic regions containing all exons and relevant exon-intron boundaries in IL1R1 and IL1R2,38 SNPs were identified in 48 subjects. Five were located within coding elements, 2 in the 5' flanking region,22 within introns, and 9 in the 3' untranslated region. Frequency of 3 SNPs and 3 haplotypes in IL1R2 showed significant differences between patients with AgP and controls (p<0.05). (3)Gingival fibroblasts were isolated from chronic periodontitis patients. And cells were stimulated with Actinobacillus actinomycetemcomitans lipopolysaccharide, which is strongly associated with AgP. IL-1β and IL-1Rt2 mRNA gene expression were measured. However, protein production was only measured for IL-1β and not for sIL-1Rt2 in culture supernatant. Project is now undergoing for different experimental design using immunocompetent cell such as neutrophil or mononuclear cells. Less