Channel-Transporter Correlation

通道和/或运输商对视图的物理学、分子生物学和生物化学点的结构和功能相关性进行了调查。在这三年里，新频道（超过29个）和新的运输公司（超过42个）被发现，他们的基因被克隆了。结构-功能相关性是通过识别功能域和氨基酸位点的数字来实现的。通道和转运器之间的功能或结构相关的新类型（超过10个）。新的疾病已被识别为通道或运输者的基因突变（超过10）。特别是，在ATP-绑定盒（ABC）转运蛋白的研究中取得了重大进展。例如，一种新型的ABC蛋白CMOAT被克隆并被发现以介导的ATP-依赖于glutathy-conjugate的传输。在都柏林-约翰逊综合症中发现了一个有缺陷的cMOAT表达式。ATP-sensitive K1 + K1 channel was revealed to be a complex of two subunits ： SUR1 of the ABC protein f\and Kir6.2 of the inward-rectifier K1 + K1 channel f\。一些ABC蛋白质已经被发现比运输机、渠道及其调节器的一项活动更多。例如，发现P-糖蛋白质和CFTR是体积敏感的Cly-D1通道和ATP释放的调节器，尊重。

Structural and functional correlations of channels and/or transporters were investigated from physiological, molecular biological and biochemical points of view. During these three years in this study, new channels (over 29) and new transporters (over 42) were discovered, and their genes were cloned. Structure-function correlations were elucidated by identification of numbers of functional domains and amino acid sites. New types of functional or structural correlation between channels and transporters were also demonstrated (over 10). New diseases were identified to be due to mutations of genes of channels or transporters (over 10). Especially a big progress was achieved in the research of ATP-binding cassette (ABC) transporter proteins. For example, a novel ABC protein cMOAT was cloned and found to mediate the ATP-dependent transport of glutathion-conjugates. The expression of cMOAT was found to be defective in the Dubin-Johnson syndrome. The ATP-sensitive KィイD1+ィエD1 channel was revealed to be a complex of two subunits : SUR1 of the ABC protein family and Kir6.2 of the inward-rectifier KィイD1+ィエD1 channel family. Some of ABC proteins have been found to have more than one activities of transporter, channel and their regulator. For example, P-glycoprotein and CFTR were found to be a regulator for volume-sensitive ClィイD1-ィエD1 channel and ATP release, respectively.