Study on the early process (especially adhesive and invasive process) of pathogenesis of bacterial enteropathogens and its cellular response

(1)肠致病性E.大肠杆菌（EPEC，EHEC）：我们发现，coronin和talin与效应物注射的vir III型分泌系统（TTSS）结合，这种结合是引起EPEC和EHEC形成的附着和消退病变所必需的。我们还发现，形成TTSS针尖的EspB也结合A/E损伤形成所必需的连环蛋白的宿主分子。(2)关于副溶血性弧菌的结果：该微生物最重要的毒力因子是TDH。/ TRH（一种外毒素），然而，这可能不足以完全理解发病机制。完成的全基因组分析表明，这有两套TTSS。TTSS-1存在于大染色体中，并分泌具有凋亡细胞毒性的效应物。另一方面，仅在临床分离株中发现的TTSS-2定位于小染色体上，可能涉及到可能具有肠道致病性的未知效应子。(3)产碱普罗威登斯菌的结果：我们首次发现了归因于产碱普罗威登斯菌（PA）的暴发。我们还发现这种微生物在一个大质粒上具有TTSS。如上所示，与我们推测这3种病原体可能具有不同的致病机制不同，很明显，这3种病原体具有共同的过程，TTSS，这可能是重要的毒力因子之一。

(1) Result on enteropathogenic E. coli (EPEC, EHEC) : We found that cortactin and talin bind to Tir, an effector injected vir Type III secretion system (TTSS) and the binding is essential for causing attaching and effacing lesion formed by EPEC and EHEC. We also found that EspB forming tip of needle of TTSS also bind host molecule of a catenin which is essential for the A/E lesion formation.(2) Result on Vibrio parahaemolyticus : The most important virulence factor of this organism is TDH../TRH (an exotoxin), however, this may not be enough to full understand of pathogenesis. Whole genome analysis completed suggested us that this has two sets of TTSSs. TTSS-1 is present in large chromosome and secrete an effector having apoptotic cytotoxicity. On the other hand, TTSS-2 found in only clinical isolates is locate on small chromosome and unidentified effector which may have enteropathogenicity may be imvolved.(3) Result on Providencia alcalifaciens : We have found, for the first time, an outbreak attributed to P alcalifaciens (PA). We also found that this organism possess TTSS on a large plasmid.. As shown above and unlike our speculation that these 3 pathogens may have different mechanism for causing diseases, it became clear that the 3 pathogens possess a common process, TTSS, which may serve as one of the important virulence factor.