Development of a light detection system for enhanced scanning electron microscopy imaging of fully hydrated biological specimens at room temperature.

该项目旨在开发仪器和技术，以在环境扫描电子显微镜中在室温下长时间成像完全水合的细胞。多个荧光纳米颗粒标记将用于以超高空间分辨率对复杂的细胞结构和动态进行成像。这种创新的生物成像技术将通过构建高速光收集系统来促进，该系统可以在稳定细胞内的水所需的压力下有效地操作。还将探讨这种新的生物成像技术的应用。

This project aims to develop instrumentation and techniques to image fully hydrated cellS for prolonged periods at room temperature in an environmental scanning electron microscope. Multiple fluorescent nano-particle labels will be used to image complex cellular structure and dynamics at ultra high spatial resolution. This innovative bio-imaging technique will be facilitated by the construction of a high speed light collection system that can efficiently operate at the pressures required to stablise water within the cell. Applications for this new bio-imaging technique will also be explored.