Proteomic analysis of subcellular changes during apoptosis

本项目旨在利用蛋白质组学的新方法，从细胞质膜、胞浆、细胞核和胞质溶胶的水平研究细胞凋亡的机制，通过SELDI-TOF质谱新技术，将蛋白质吸附到活化的芯片上，筛选细胞器的蛋白质提取物。这将提供细胞凋亡不同阶段的蛋白质质量特征，并将允许通过常规蛋白质组学方法鉴定感兴趣的蛋白质。SELDI-MS作为细胞蛋白质组学的可行工具的建立将为在蛋白质表达水平上理解广泛的细胞功能提供新的机会。

This project aims to use a novel proteomic approach to examine mechanisms of apoptosis at the level of the plasma membrane, mitochondrion, nucleus and cytosol, screening protein extracts of cell organelles by the new technique of SELDI-TOF mass spectrometry in which proteins are adsorbed onto activated chips. This will provide protein mass profiles characteristic of various stages of apoptosis, and will allow identification of proteins of interest by conventional proteomic methods. The establishment of SELDI-MS as a viable tool for cell proteomics would open new opportunities to understand a broad range of cellular functions at the level of protein expression.