Tandem mass spectrometer coupled to an ultra-high performance liquid chromatograph: UHPLC-HRMS/MS

通过目前申请UHPLC-HRMS/MS系统的大型设备资金，生物工艺工程研究所（BVT）正在追求进一步发展的基本目标：（1）巩固酶催化领域的成功项目，（2）使用具有生物活性的天然物质，以及（3）在微生物生物技术和动物细胞培养领域开展系统生物学项目。这里描述的设备对于2018年和2022年两位新任命的教授Kathrin Castiglione（生物工艺工程）和塞巴斯蒂安Aljoscha Wahl（系统生物技术）的计划工作至关重要。该设备将能够在小分子领域进行精确分析，以识别和定量反应混合物，天然物质，培养基以及细胞中的物质（代谢组学）。此外，该设备将能够测量蛋白质组成。复杂样品中的此类复杂分子的测量只能通过高分辨率、高灵敏度的系统来实现。采购由大学以聘用承诺的形式提供财政支持。计划研究的目的是开发和应用工具：（1）复杂的生物催化过程的调查和优化，（2）生物活性天然物质的鉴定，以制定针对病毒和细菌的自然防御策略，以及（3）（动物）细胞的系统生物学分析。该研究所提供的复杂催化，生物过程，培养，代谢和建模的知识为在HR-MS/MS的帮助下解决新的科学问题提供了一个非常好的起点。

With the present application for large-scale equipment funding for a UHPLC-HRMS/MS system, the Institute of Bioprocess Engineering (BVT) is pursuing essential goals for further development: (1) The consolidation of successful projects in the field of enzymatic catalysis and (2) the use of natural substances with biological activity, as well as (3) the development of systems biology projects in the field of microbial biotechnology and animal cell culture. The device described here is essential for the planned work of the two newly appointed professors Kathrin Castiglione (Bioprocess Engineering) and Sebastian Aljoscha Wahl (Systems Biotechnology) in 2018 and 2022. The device will enable precise analyses in the field of small molecules for the identification and quantification of substances in reaction mixtures, natural substances, media as well as in cells (metabolomics). Furthermore, the device will enable the measurement of protein composition. Measurements of such complex molecules in complex samples can only be achieved with a high-resolution, sensitive system. The procurement is financially supported by the university in the form of appointment commitments. The aim of planned research is to develop and apply tools for: (1) the investigation and optimisation of complex biocatalytic processes, (2) the identification of bioactive natural substances for the development of natural defense strategies against viruses and bacteria, and (3) the systems biology analysis of (animal) cells. The knowledge of complex catalyses, bioprocesses, cultivation, metabolism and modelling available at the institute offers a very good starting point for addressing new scientific questions with the help of HR-MS/MS.