Experimental study to facilitate the repaair of brain injury by artificial modification of cellular function and brain transplantation.

研究了局灶性缺血后损伤的修复过程，特别关注了神经胶质细胞群的增殖。探讨改善或修复缺血性脑损伤的治疗措施。研究沙鼠局灶性脑缺血后1 ~ 7天神经胶质细胞增殖的动态变化。结果表明，脑梗死后第1 ~ 5天，星形胶质细胞在脑梗死周围增殖活跃，DNA合成在第3天最为活跃。此外，这些区域被大量的多形核细胞和巨噬细胞浸润。后一类细胞似乎特别重要，因为它们的白细胞介素1 （IL-1）呈阳性。研究了小刀伤对沙鼠海马神经元死亡进化的影响。结果表明，这种轻微损伤对海马锥体神经元在短暂性缺血后的延迟死亡具有一定的保护机制。在缺血侮辱前24小时刺入海马的效果最为显著。研究了新生儿室管膜下细胞移植入成人海马后的生长分化情况。这些细胞以有限的方式增殖了几周，并分化为神经胶质细胞，但不是神经元。我们进一步研究了胎儿室管膜下细胞的移植，其中大量细胞分化为神经元。在短暂性前脑缺血后1 ~ 4周，将新生海马组织移植到成年沙鼠海马中。结果表明，海马梗死，特别是短暂缺血1周后，对移植神经组织存活是可行的。

The repair process of injury after focal ischemia was studied with special reference on the proliferation of glial population. Therapeutic measure was then searched for to ameliorate or repair the ischemic brain injury.1. The dynamic profile of proliferation of glial cells was studied 1 to 7 days after focal cerebral ischemia in gerbils. The results indicated that the astrocytes proliferated actively at the periphery of infarcted brain during days 1 to 5 postinfarction with most active DNA synthesis at day 3. Additionally, those regions were infiltrated with large number of polymorphonuclear cells and macrophages. The latter type of cells seems especially important since they were positive for interleukin 1 (IL-1).2. Effect of preceding small stab wound on the evolution of hippocampal neuronal death was studied in gerbils. The results indicated that a certain kind of protective mechanism was induced by this type of minor injury to save hippocampal pyramidal neurons from delayed death after transient ischemia. The effect was most impressive when the hippocampus was stabbed 24 hours before ischemic insult.3. The growth and differentiation of transplanted neonatal subependymal cells was studied after transplantation into adult hippocampus. Those cells proliferated for a few weeks in a limited fashion, and differentiated to glial cells but not neurons. We further studied transplantation of fetal subependymal cells, a substantial number of which differentiated to neurons.4. The neonatal hippocampal tissue was transplanted into the hippocampus of adult gerbils at 1 to 4 weeks after transient forebrain ischemia. The results demonstrated that the infarcted hippocampus, especially 1 week after transient ischemia, was feasible for survival of transplanted neural tissue.