Immunocytochemical analysis on localization of plant hormones in callus related to regeneration capaci

为明确愈伤组织再生能力与激素定位的关系，建立了水稻细胞悬浮培养不定芽发生和胚状体发生的培养体系。将水稻愈伤组织转移到含有低浓度激动素和阿坝的再生培养基上，胚状体分化率高。另一方面，当将其转移到含有高水平1-（2-氯-4-吡啶基）-3-苯基脲（一种细胞分裂素）而不是激动素的培养基上时，不定芽的分化率高。为了检测IAA和阿坝的定位，我们用抗IAA和抗ABA的单克隆抗体对水稻离体培养体系进行了免疫细胞化学检测。IAA和阿坝均不能被检测到，因为表位位点被认为被固定处理破坏。

To clear the relation between regeneration capacity and localization of plant hormones in callus, a culture system of adventitious shoot formation and embryogenesis from cell suspension of rice (Oryza sativa L.) was established and immunocytochemistry was applied.When rice callus was transferred onto a regeneration medium containing low level of kinetin and ABA, it differentiated embryoid in high rate. On the other hand, when it was transferred onto a medium containing high level of 1-(2-chloro-4-pyridyl)-3-phenylurea (a kind of cytokinin) instead of kinetin, it differentiated adventitious shoots in high rate. Then, it is clear that regeneration pathway can be controlled by culture conditions.To detect IAA and ABA localization, immunocytochemistry was applied to the rice culture system using anti-IAA or anti-ABA monoclonal antibody. Neither IAA nor ABA could be detected, because epitope sites considered to be broken by fixation treatment.