Study of Experimental Oral Tumors Induced by Mutagenic Heterocyclic Amines in Boiled Food

本研究旨在从组织学和超微结构的角度研究二次创伤后经色氨酸-P-2(3-氨基-L-甲基-5H-吡啶并[4，3-b]吲哚)处理的舌上皮细胞的变化。光镜下将仓鼠分为10组(A~J组)。10组均用剪刀切除舌尖(2 Mm)。A、F组创面涂抹二甲基亚砜(DMSO)，每周三次，共8周。B、C、D、G、H、I组用0.5%色氨酸-P-2二甲基亚砜溶液涂抹创面，每周三次，共8周。E组和J组创面未处理8周。8周末，A、B、C、D、E组再次切除舌尖，F、G、H、I、J组不切除舌尖。A、C、F、H组每日舌上涂DRSO，直至动物被处死。在B组和G组，动物在被杀之前没有接受任何治疗。D组、E组、L组和J组每日涂抹…更多的是使用Trp-P-2，直到动物被杀。各组动物在第二次切除后11天、15天或8周结束后处死动物。对舌头进行组织学检查。A、c、d组动物分别接受与A、C、D组相同的处理。这些动物在第二次切除后15天被处死。对舌头进行超微结构检查。光镜观察1)A组第11天和第15天舌象仅见角化过度。2)B组11天、15天舌象雪白，轻度上皮异型增生。3)C组和D组在第11天和第15天时，舌上皮细胞出现明显的异型增生改变。这些改变包括细胞定向障碍、细胞和核的多形性、核深染、有丝分裂样增多、角质化异常和上皮突起延长。4)E组11天、15天时舌象上皮轻度异型增生改变。5)F、G、H、I、J组舌象仅表现为过度角化。电子显微镜观察：1)A组基底细胞层细胞呈圆形或卵圆形，胞核大而集中。基底细胞正下方的基底层几乎完好，但有时缺乏或不规则的多层。棘细胞层中的细胞稍扁平。这些细胞胞质中的张力细丝束包绕核或朝向桥粒。颗粒细胞层细胞中度扁平，内含不同数量的角质层透明颗粒。角化细胞层中的细胞非常扁平，无核。2)c组和d组基底细胞层细胞形态不规则。细胞核增大，形状不规则。胞质中的张力细丝束环绕胞核或呈漩涡状排列。偶见核内可见管状结构。基细胞下方的基膜常无或不规则多层。棘细胞层中的细胞形状不规则。在这些细胞的细胞质中，张力细丝束绕在细胞核周围，或散在或局限，或呈漩涡状排列。偶见核内可见病毒样颗粒和核内管状结构。颗粒细胞层细胞扁平。有时，这些细胞的细胞核中含有病毒样颗粒。角化细胞层中的细胞通常没有核，但有时有一个带有病毒样颗粒的核。较少

The purpose of this study was to investigate histologically and ultrastructurally the changes of epithelial cells in the tongue which was wounded twice and treated with Trp-P-2(3-amino-l-metliyl-5H -pyrido[4,3-b]indole).1. Light Microscopy Hamsters were divided into 10 groups(Groups A-J). In all 10 groups, the tip(2mm) of the tongue was excised with scissors. In Groups A and F, the wound was painted trice weekly with DMSO(dimethyl sulf- oxide) for 8 weeks. In Groups B,C,D,G,H and I, the wound was painted trice weekly with a 0.5%solution of Trp-P-2 in DMSO for 8 weeks. In Groups E and J, the wound was not treated for 8 weeks. At the end of 8 weeks, in Groups A,B,C,D and E, the tip of the tongue was again excised, while in Groups F,G,H,I and J, the tip was not excised. In Groups A,C,F and H, the tongue was painted daily with DRSO until the animals were killed. In Groups B and G, the animals received no treatment until they were killed. In Groups D,E,l and J, the tongue was painted daily … More with Trp-P-2 until the animals were killed. Animals of all groups were killed aL 11 and 15 days after the second excision or after the end of 8 week. The tongue was examined histologically.2. Electron Microscopy In Groups a,c and d, the animals received the same treatment as those of Groups A,C and D, respectively. The animals were killed at 15 days after the second excision. The tongue was examined ultra- structurally.3. Light Microscopic Observation 1)In Groups A, at 11 and 15 days, the tongue showed only hyperkeratosis. 2)ln Groups B, at 11 and 15 days, the tongue snowed the changes of the mild epithelial dysplasia. 3)ln Groups C and D, at 11 and 15 days, the tongue showed the changes of the marked epithelial dysplasia. These changes were disorientation of cells, cellular and nuclear pleomorphisms, nuclear hyperchromatism, increased number of mitotic figures, abnormal keratinization and elongation of epithelial projection. 4)In Group E, at 11 and 15 days, the tongue showed the changes of the mild epithelial dysplasia. 5)In Groups F,G,H,I and J, the tongue showed only hyperkeratosis.4. Electron Microscopic Observation 1)In Group a, the cells in the basal cell layer were round or ovoid in shape with large centrally-located nuclei. The basal lamitia just below the basal cells was almost intact, but on occasions absent or irregularly multilayered. The cells in the prickle cell layer were slightly flattened. The bundles of tonofilaments in the cytoplasm of these cells surrounded the nucleus or were oriented towards the desmosomes. The cells in the granular cell layer were moderately flattened and contained various amount of keratohyaline granules. The cells in the keratinized cell layer were very flattened and had no nucleus. 2)In Groups c and d, the cells in the basal cell layer were irregular in shape. The nucleus was enlarged and also appeared irregular in shape. The bundles of tonofilaments in the cytoplasm surrounded the nucleus or showed swirl-like arrangement. Occasionally, intranuclear tubular-like structures were observed within the nucleus. The basal lamina below the basal cells frequently absent or irregularly multilayered. The cells in the prickle cell layer were irregular in shape. In the cytoplasm of these cells, the bundles of tonofilaments surrounded the nucleus or scattered or localized or showed a swirl-like arrangement. Occasionally, virus-like particles and intranuclear tubular-like structures were observed within the nucleus. The cells in the granular cell layer were flattened. Sometimes, the nuclei of these cells contained virus-like particles. The cells in the keratinized cell layer usually lacked a nucleus, but on occasions had a nucleus with virus-like particles. Less