Studies on dynamic changes of molecular distribution in plant cells by immuno-electron microscopy

我们开发了一种免疫光电子显微镜，并检测了植物细胞中DNA和蛋白质的动态变化。因此，我们获得了关于这些问题的新数据。特别是，Osafune和Ueda改进了免疫金电子显微镜（IGEM），以获得高分辨率。这些新开发的技术被应用于包括该小组在内的研究人员的各个领域。Kuroiwa开发了一种光显微镜和电子显微镜同时观察的技术，并应用该技术分析了天竺葵（Pelargonium zonale）、烟草（Nicotiana tabacum）和拟南芥（Arabidopsis thaliana）根分生组织细胞的增殖。结果表明，在mtDNA合成活跃的静止中心上方的细胞中，每mt-细胞核的mtDNA含量约为3000千碱基对，而在根伸长区下方的分生细胞中，mtDNA含量低于170千碱基对。Murakami利用IGEM证明光系统I位于类囊体膜系统中，而光系统II位于基质类囊体中。Osafune通过高分辨率IGEM发现LHCPII载脂蛋白通过高尔基体转移到质体上。Ueda表明肌动蛋白位于Trebouxia溶酶体表面。Tanaka， Yamaguchi和Miyakawa分别揭示了酵母细胞中蛋白酶的行为，病毒样颗粒的形成和线粒体核蛋白的存在。

We have developed an immuno-light-electron microscopy and examined the dynamic changes of DNA and proteins in plant cells. As a result, we have obtained new data about these problems. Especially, Osafune and Ueda improved the immuno-gold-electron microscopy (IGEM) to obtain a high resolution. The newly developed techniques are applied to various fields of these investigators including the group. Kuroiwa developed a technique for the simultaneous observations of light microscopic field and electron microscopic field and applied the technique to analyzing the multiplication of cells in root meristems of Pelargonium zonale, Nicotiana tabacum and Arabidopsis thaliana. The results showed that the mtDNA content per mt-nucleus in the cells just above quiescent center, where the synthesis of mtDNA is active, corresponds to approximately 3000 kilobase pairs but, in the meristematic cells just below the elongation zone of the root it falls less than 170 kbp. Murakami showed using IGEM that the photosystem I is located in thylakoid membrane systems, while the photosystem II in stroma thylakoids. Osafune found by a high resolution IGEM that LHCPII apoprotein transferred to plastid through the Golgi apparatus. Ueda showed that the actin is located on the surface of lysosomes in Trebouxia. Tanaka, Yamaguchi and Miyakawa revealed the behavior of proteinase, the formation of virus-like particles and the existence of mitchondorial nuclear proteins in yeast cells, respectively.