Production of Interspecific Hybrids between kiwifruit and Relted Species by Ovary Slice Culture

1.猕猴桃及其近缘种子房切片培养植株的诱导接种后14d左右，在100g/l蔗糖的条件下，可识别出白色种子。将种子转入MS盐+30g/l蔗糖+8g/l琼脂+玉米素1.0 mg/1.2的植株诱导培养基中。猕猴桃及其近缘种的过氧化物酶同工酶变异用等电法研究了过氧化物酶同工酶带型在猕猴桃及其近缘种中作为基因标记的作用。采集了4个猕猴桃品种(A.delisiosa)、6个Sarunashi品种(A.garta)、1个Matatabi品种(A.polgama)和1个Shimasarunashi品种(A.rufa)的叶片样品。美味曲霉、软曲霉、多年生黑曲霉和红曲霉的过氧化物酶同工酶谱带有显著差异。不同栽培品种间过氧化物酶同工酶谱带无明显差异。

1. Induction of plants from ovary slice culture in kiwifruit and related speciesOvaries of sarunashi(A.arguta), sampled 5-10 days after pollination, were sliied into disks 1-2mm thick. About 14 days afterin oculation, white seeds were recognized, with 100g/1 sucrose medium. In A.arguta(2n=116) x A.arguta(2n=116), seed were transferred on plantet-inducing medium, which consisted of MS salt supple-men ted with 30g/1 sucrose, 8g/1 agar and zeatin 1.0mg/1.2. Peroxidase isozyme variations in kiwifruit and related speciesThe usefulness of peroxidase isozyme banding patterns as gene-tic markers in kiwifruit and related species was investigated using isoelectric forcusing. Leaf samples were obtained from four cultivars of kiwifruit(A.delisiosa), six varieties of sarunashi( A.arguta), one variety of matatabi(A.polygama) and one variety of shimasarunashi(A.rufa). There were marked differences in the peroxidase zymogram patterns between A.deliciosa, A.arguta, A.pol-ygama and A.rufa. No difference of zymogram patterns of peroxidase was observed among culitivars of A.deliciosa.