Analysis of immunoregulatory anti-idiotypic antibodies during human schistosomiasis japonica.

我们分析了抗独特型T细胞反应在人日本血吸虫病中的免疫学作用。慢性患者的血清诱导来自既往感染个体的T细胞显著增殖。应答细胞CD3和CD4呈阳性，cd8呈阴性。这些T细胞产生IFN-作为对感染血清的反应，而不产生可检测到的IL-4和IL-5。提示Th1细胞对血吸虫卵肉芽肿过敏反应具有反应性，而Th2细胞对血吸虫卵肉芽肿过敏反应具有拮抗作用。血清因子(s)见于IgG部分，但与抗血吸虫卵抗体不同。在某些情况下，慢性血清诱导了已建立的日本血吸虫卵抗原特异性T细胞系的应答，因此血清因子极有可能是抗独特型IgG识别抗s抗体。日本血吸虫卵抗原。这种抗独特型抗体仅在新患者中观察到，而在治疗超过7年的个体中未观察到活性。当我们比较“新鲜”和“旧”血清对HPLC分离的鸡蛋抗原的反应性时，只有“新鲜”患者血清才能识别出400kD的部分。抗血吸虫IgG特异性的差异可能影响人感染日本血吸虫不同阶段的抗独特型抗体反应。

We analyzed immunological roles of anti-idiotypic T cell responses during human schistosomiasis japonica. Sera of chronic patients induced significant proliferation of T cells from individuals of previous infection. The responding cells were positive for CD3 and CD4, but were CD8-negative. Those T cells produced IFN- in response to the infected sera, whereas no detectable IL-4 and IL-5 were produced. This suggested that Th1 cells were reactive to the sera, and those could be antagonistic to Th2 cells which were tightly involved in granulomatous hypersensitivity to schistosome eggs. The serum factor(s) was observed in IgG fraction, but was different from an anti-schistosome egg antibody. In some cases chronic sera induced responses of established T cell lines specific for Schistosoma japonicum egg antigen, and it was thus highly likely that the serum factor was anti-idiotypic IgG recognizing antibodies to anti-S. japonicum egg antigen.Such anti-idiotypic antibodies were observed only in fresh patients, however, no activity was observed in individuals who had been treated more than 7 years before. When we compared the reactivities of "fresh" and "old" sera to the egg antigen fractionated by HPLC, a 400kD fraction(s) was recognized only by "fresh" patient sera. The difference in specificity of anti-schistosome IgG seemed to have influence on anti-idiotypic antibody responses in various infection phases during human infection of S. japonicum.