Development of artificial seed using plant hairy root

从生物工程的角度出发，研究了利用人工种子系统进行辣根毛状根微繁的方法。1. 生长素的补充促进了茎尖分生组织数量的增加和细胞生长速率的提高。建立了毛状根生长动力学模型。2. 在黑暗条件下，被叶片切除的根碎片和根尖分生组织再生能力强。通过显微形态学观察和光照下植株的变化，鉴定了辣根毛状根上的不定根原基。与其他两种细胞相比，不定芽原基在光照下的发育具有相对较高的同步性。在人工种子系统中，有必要增加不定芽原基形成的数量。4. 通过补充细胞分裂素，可获得毛状根发育成的植株。为了把根切成碎片，使用了搅拌器。机械切除改善了生物过程，产生了能胜任的根片段作为人工种子。生物反应器的再生频率与摇瓶中的再生频率相近。将包含在微珠中的候选毛根细胞分为3类；1、根片在黑暗条件下培养。2、在黑暗条件下形成不定芽原基。移栽后形成的毛状根发育成植株。上述结果表明了毛状根在人工制种中的应用可能性。

We focused on the development of micropropagation procedure of horseradish hairy root by using artificial seed system from the standpoint of bioengineering. 1. Auxin supplementation stimulated branching, which resulted in the increment of the number of apical meristem and cell growth rate. The kinetic growth model was developed to simulate the hairy root growth. 2. The root fragments with branch or apical meristem excised by blade were highly regenerated under dark conditions. 3 The adventitious shoot primordium on the horseradish hairy root by microscopic morphological observation and alteration to plantlet at the exposure to light is identified. The adventitious shoot primordium offers an advantage for relatively higher synchrony in development at exposure to light, compared with two other cells. It is necessary to increase the number of the adventitious shoot primordium formation in an artificial seed system. 4. Plantlet developed from hairy root were obtained by treatment cytokinin supplementation. To cut the root into fragments, the blender was used. The mechanical excision improved the bioprocess to produce the competent root fragments as artificial seed. the bioreactor, the regeneration frequency was similar to that of that in shake flask. The candidate hairy root cells included in beads were classified into three ; 1, the root fragment cultured under dark condition. 2, the adventitious shoot primordium formed under dark condition. 3, plantlet developed from the hairy root which was formed after transferred into light. The above results show the possibility of application of hairy root for artificial seed production.