Development of foam countercurrent chromatography for application works

最近，通过开发泡沫逆流色谱（Foam CCC）实现了泡沫分离技术的创新，其已成功地应用于使用氮气和不含表面活性剂或其它添加剂的蒸馏水分离和富集样品中的疏水组分。结果表明，该方法可有效地应用于泡沫活性组分的分离富集。为了便于将该技术应用于各种天然产物，非常希望引入一组物理化学参数，其可靠地指示样品对泡沫CCC的适用性，并开发样品注入方法，通过该方法可以将大量的样品溶液引入到泡沫CCC中。为了简单地测试泡沫CCC对样品的适用性，作者引入了两个参数，I.例如，“发泡力”和“泡沫稳定性”。在每次试验中，将样品放入量筒中， ...更多信息 呃接着就是剧烈的摇晃。起泡力用所得泡沫与剩余溶液的体积比表示，泡沫稳定性用泡沫持续时间表示。从杆菌肽、栀子黄、荧光桃红B和玫瑰红等不同样品中获得的一组数据显示，这些参数与泡沫CCC的泡沫富集程度之间存在显著相关性。泡沫稳定性在250 min以上。对于进样方法，开发了一种新的系统--“循环进样系统”。该系统在将大量杆菌肽溶液引入泡沫CCC中的实验中得到了证实，疏水组分杆菌肽A几乎从原始样品溶液中分离并富集在泡沫部分中。因此，该循环进样系统可实现杆菌肽复合物疏水组分的分离富集。该方法将是非常有效的分离和富集的化合物从粗天然产物。少

Recently, an innovation of the foam separation technology has been achieved by development of foam countercurrent chromatography (Foam CCC), which has been successfully applied to separation and enrichment of hydrophobic components in a sample using nitrogen gas and distillled water free of surfactant or other additives. The results indicated that the method may be effectively applied to separation and enrichment of foam active components. In order to facilitate the application of the technique to various natural products, it is highly desirable to introduce a set of physicochemical parameters which reliable indicate applicability of a sample to the foam CCC and to develop a sample injection method, by which a large volume of the sample solution can be introduced into the foam CCC.To simply test the applicability of foam CCC to a sample the authors have introduced two parameters, i. e., "foaming power" and "foam stability". In each test, the sample was delivered into a graduated cylind … More er followed by vigorous shaking. The foaming power was expressed by the volume ratio of the resulting foam to the remaining solution, and the foam stability by the duration of the foam. A set of data obtained from various samples including bacitracin, gardenia yellow, phloxine B, and rose bengale revealed a significant correlation between these paramenters and the degree of foam enrichment by foam CCC.The overall results indicated that the sample with the following properties may be effectively enriched by foam CCC : the foaming power is greater than 1.0 and the foam stability is over 250 min.For the sample injection method, a new system named "recycle injection system" has been developed. The utility of this system was demonstrated in the introduction of a large volume of bacitracin solution to the foam CCC.A hydrophobic component, bacitracin A, has been almost isolated and enriched in the foam fraction from the original sample solution. Thus, satisfactory separation and enrichment of the hydrophobic components of bacitracin complex could be obtained by this recycle injection system. The method would be very effective for separation and enrichment of a compound from crude natural products. Less