Advanced lattice light sheet microscope optimised for biological imaging

该项目提供了一种格子光片显微镜，该显微镜优化了生物应用和用户实用。晶格光片显微镜结合了光学、物理和技术属性，以最小的光毒性实现了对生物标本的长时间高时空分辨率成像，首次实现了对活标本的高分辨率纵向体积分析。它适用于细胞和亚细胞分辨率的培养细胞、器官、小胚胎和斑马鱼幼体等小型模式生物的成像。预期的结果是高影响力的发现、培训机会、国际合作和解决生物学基本问题的出版物。

This project provides a lattice light sheet microscope optimised for biological applications and user utility. Lattice light sheet microscopy combines optical, physical and technical attributes that achieve high spatio-temporal resolution imaging of biological specimens for long periods with minimal phototoxicity, making practical for the first time high-resolution longitudinal volumetric analyses of living specimens. It is suitable for imaging cultured cells, organoids, small embryos, and small model organisms such as zebrafish larvae at cellular and subcellular resolution. Expected outcomes are high impact discoveries, training opportunities, international collaborations and publications addressing fundamental questions in biology.