The immunologically and molecular biologically investigation for diagnostic methods of periodontal disease activity

本研究旨在从免疫学和分子生物学角度评价牙周炎的病情，并开发诊断活动性病变和易感患者的方法。介绍了应用DNA探针和聚合酶链反应技术对龈下菌斑中牙周致病菌进行敏感检测和鉴定的方法。结果表明，牙龈卟啉单胞菌（Pg）常在活动性病变中检出。牙周治疗失败的病变组织中常检出齿垢毛癣菌（Td），而治疗成功的病变组织中未检出，提示Td可作为牙周治疗后病变组织病变程度的诊断指标。还表明V γ 9/V δ 2 T细胞可能在预防细菌感染或组织破坏中起重要作用。此外，针对热休克蛋白（HSP）60的免疫反应可能会与牙周炎相关微生物和宿主细胞发生反应， ...更多信息 牙周炎患者的HSP 60特异性抗体与牙龈成纤维细胞分离的蛋白质有交叉反应。此外，牙周病易感者血清中抗Pg抗体水平明显高于耐药者，提示血清中Pg抗体水平可作为牙周病进展的危险指标。龈沟液中多胺、碱性磷酸酶、血小板活化因子活性、中性粒细胞溶酶体蛋白酶、分泌性白细胞蛋白酶抑制剂、炎性细胞因子及前列腺素E_2水平均较高。此外，牙周袋的pH值显着高的深探测深度的网站。上述参数对活动性牙周病的诊断有一定的参考价值。总之，结合本研究中检查的局部病变部位的宿主免疫反应、炎性细胞因子和介质，可以使我们能够诊断牙周剂量酶活性。少

This study was designed to immunologically and molecular biologically evaluate the diseased conditions of periodontitis and develop procedures to diagnose active lesions and susceptible patients. The techniques utilizing DNA probes and polymerase chain reaction were introduced to sensitively detect and identify periodontopathic bacteria in subgingival plaque. It was shown that P.gingivalis (Pg) was frequently detected in active diseased lesions. Additionally, T.denticola (Td) was always found in the unsuccessfully treated periodontal lesions but not in the successfully treated lesions, suggesting that Td would be a diagnostic indicator of the diseased conditions in the lesions after periodontal treatments. It was also suggested that Vgamma9/Vdelta2 T cells may play an important role in prevention of bacterial infection or tissue destruction. Futhermore, immune response against heat shock protein (HSP) 60 may react with both periodontitis-associated microorganisms and host cells because … More HSP 60 specific antibodies of periodontitis patients crossreact to proteins isolated from gingival fibroblast. In addition, it was also indicated that the levels of serum actibodies specific to Pg could be used as a risk indicator for the progression of periodontal diseases, because the susceptible patients to periodontal diseases exhibited significantly higher anti-Pg antibody levels than the resistant patients. Furthermore, the high levels of polyamines, alkaline phosphatase, platelet-activating factor activities, neutrophil lysosomal proteinase (medullasin and cathepsin G), secretory leukocyte protease inhibitor (SLPI), inflammatory cytokines (IL-1, IL-6, Il-8) and prostaglandin E_2 were frequently observed in gingival crevicular fluid. Also pH in periodontal pockets was significantly high in the sites with deep probing depth. Parameters showing above seem to be useful to diagnose active periodontal diseases. In conclusion, it was suggested that the combining host immune responses, inflammatory cytokines and mediators in local diseased sites examined in this study may enable us to diagnose periodontal dosease activity. Less