STUDIES ON HUMAN PROSTATE TISSUE CONFORMATION AND INTER-CELLULAR REGULATORY SYSTEM

一种系统内分泌因子，雄激素，打开前列腺局部控制系统的开关，诱导前列腺的发育和增殖。本研究的目的是探讨局部因素如何与前列腺内分泌环境的操纵引起的前列腺变化有关。在一九九七年至一九九八年的拨款资助下，我们对本地控制系统的下列标记进行了研究。前列腺上皮功能（前列腺特异性抗原；PSA）：前列腺增生和癌症患者的前列腺免疫组织化学PSA染色在接受雄激素阻断治疗的患者血清PSA水平降低时减弱。与增殖相关的因素（c- erbb -2, ki-67）：在雄激素阻断治疗的患者中，前列腺中ki-67抗原的免疫组织化学染色也普遍下降，但在雄激素阻断抵抗或低分化癌症患者中没有。我们无法获得c- erbb -2.3的明确结果。凋亡（核内DNA片段化）和凋亡抑制剂（bcl-2）：细胞凋亡局限于腺泡上皮细胞，bcl-2存在于导管上皮细胞和基底细胞。雄激素阻断治疗后BPH和癌组织中细胞凋亡增加。而Bcl-2在凋亡中表达差异。大鼠的细胞凋亡在去势后3 d达到最大值，此后逐渐减少。与凋亡相比，bcl-2的表达延迟并逐渐升高。现在，我们计划扩展我们的研究，包括tgf - β等负生长控制因子，以及更详细的组织构象关系。

A system endocrine factor, androgen, turns on the switch of the local control system of the prostate gland to induce development and proliferation. The aim of the present project was to investigage how the local factors were related to the changes of the prostate gland provoked by the manipulation of the endocrine environment of the prostate. Being supplied with the grant from 1997 to 1998, we conducted studies on the following markers of the local control system.1. Prostate epithelial function (prostate specific antigen ; PSA) : Immunohistochemical PSA stain in the prostate gland both in BPH and cancer, was weakened in patients undergoing androgen blockade treatments in whom serum PSA levels were decreased.2. Factors related to proliferation (c-erb-2, ki-67) : Immunohistochemical stain of ki-67 antigen in the prostate was also decreased in general in patients with androgen blockade treatment, but not in these with androgen blockade resistant or poorly differentiated cancer. We could not obtain definitive results on c-erb-2.3. Apoptosis (intranuclear DNA fragmentation) and apoptosis inhibitor (bcl-2) : Apoptosis was localized in the acinar epithelial cells, while bcl-2 was detected in the duct epithelial cells and basal cells. Apoptosis increased following androgen blockade treatments both in BPH and cancer tissue. Bcl-2, however, differentially expressed from apoptosis. In rats, apoptosis reached maximum 3 days aftercastration and thereafter decreased gradually. Expression of bcl-2 was delayd compared to apoptosis and increased gradually.Now, we are planning to extend our studies covering negative growth control factors such as TGF-beta, and more detailed relation to tissue conformation.