Study on the automated DNA profilling in paternity testing

根据核心重复序列的长度和基因组分布，将两个系统分为小卫星和微卫星(或短串联重复)。特别是，短串联重复序列(STR)是一类分布于整个基因组的丰富的多态基因座。每个STR基因座通常由2-5个碱基对的有限数量的核心重复序列组成，这一点很容易通过分析凝胶电泳来分辨。对日本和中国人群进行了ACTBP2和D4S43基因座的研究。结合使用不同荧光染料的荧光标记引物，等位基因大小范围相同或重叠的STR基因座可以在同一条聚丙烯酰胺凝胶中同时进行分析，从而为亲子鉴定和人类鉴定提供了一种快速、准确的方法。基于荧光的自动DNA填充技术及其应用已经被研究并被验证用于亲子鉴定例行程序。

Based on the length and genomic distribution of the core repeat sequences, two systems are classified as minisatellites and microsatellites (or short tandem repeats). In particular, short tandem repeats (STRs) are abundant classes of polymorphic loci dispersed throughout the entire genome. Each STR locus usually consists of a limited number of the core repeats of 2-5 basepairs, which can easily be resolved by analytical gel electrophoresis. The Japanese and Chinese populations were studies for the ACTBP2 and D4S43 loci. In combination with the use of fluorescence-labeled primers employing different fluorescent dyes, STR loci with overlapping or identical allele size ranges can be analyzed together in the same lane of polyacrylamide gel, thus providing a rapid and accurate method for paternity testing and human identification. Automated DNA profilling by fluorescence-based technology, as well as its application, have been studied and validated for use in paternity testing routine.