A study for establishment of judging periodontal disease activity

本研究的目的是阐明IFN-γ和IL-4在牙周病中的作用。在检查临床参数后，在拔牙或牙周手术时对牙龈进行活检。获得连续石蜡切片，并且每五个切片用抗IFN-γ或IL-4单克隆抗体进行免疫组织学染色。标本分为三组：拔牙时无治疗（NT），牙周手术时深袋治疗1（T1），浅袋治疗2（T2）。IFN-γ阳性细胞数NT最多，T2次之。当标本与探诊出血（Bop）分组时，Bop+中IFN-γ携带细胞的数量较高。炎症细胞浸润较弱的牙龈组织中IFN-γ阳性细胞数明显高于炎症细胞浸润较强的牙龈组织。而IL-4阳性细胞则相反。提示IFN-γ与牙周病的活动有关，IL-4在牙周病活动中起抑制作用。

The purpose of this study was to clarify the role of IFN-gamma and IL-4 in periodontal disease. Gingiva was biopsied at tooth extraction or periodontal surgery after examining clinical parameters. Serial paraffin sections were obtained and every five sections were stained immunohistologically with anti-IFN-gamma or IL-4 monoclonal antibody. The specimens were divided into three groups ; no treatment (NT) obtained at tooth extraction, treatment 1 (T1) obtained at periodontal surgery with deep pocket, and treatment 2 (T2) with shallow pocket. Then the number of IFN-gamma bearing cells was highest in NT and that in T2 was the next. When the specimens were grouped with bleeding on probing (Bop), the number of IFN-gamma bearing cells was higher in Bop+. Furthermore, the number of IFN-gamma bearing cells was higher in the gingiva with strong inflammatory cell infiltration than that with weak one. On the other hand, IL-4 bearing cells showed opposite tendency. These findings suggested that IFN-gamma is related with activation of periodontal disease and IL-4 plays the suppressive role in periodontal disease activity.