Correlation between Apoptosis, oncogene, SOD and radiation response

1 Bax、bcl-2蛋白表达与放射诱导细胞凋亡的相关性研究21例宫颈癌组织中Bax、bcl-2蛋白表达与放射诱导细胞凋亡的相关性，其中2期11例，3期8例，4期2例。采用Oncor公司的Apoptag软件检测细胞凋亡，计算凋亡指数（Apoptotic Index，AI）。结果：照射前细胞凋亡率为0.02%，9 Gy时细胞凋亡率明显增加，达1.2%; Bax的剂量率在照射前为15%，9 Gy时增加到60%。而bcl-2在照射前为15%，照射9 Gy时上升至25%，但无明显增加。结论：Bax蛋白表达与宫颈癌放射诱导的细胞凋亡密切相关。2 p53、p21蛋白表达与宫颈癌放射诱导细胞凋亡的相关性研究p53、p21蛋白表达与宫颈癌放射诱导细胞凋亡的相关性。照射前p53和p21的平均AI分别为6.60%和9.24%。9 Gy后p53和p21的平均AI分别为13.9%和18.0%。照射前p53蛋白指数与p21蛋白指数呈显著正相关。平均AI、p53指数和p21指数分别为0.98%、7.33%和8.77%。辐射诱导的AI与p53、p21呈显著负相关。结论：p53和p21级联反应在宫颈癌中起凋亡抑制作用。

1 Correlation between Bax, bcl-2 protein expression and radiation-induced apoptosisBax bcl-2 protein expression correlate with radiation-induced apoptosis on 21 cases with the cervical cancer, including 11 cases of stage 2, 8 cases of stage 3, and 2 cases of stage 4 was investigated. Apoptosis was detected by using Apoptag (Oncor), and Apoptotic Index (AI) was calculated. Bax and bcl-2 protein expressions were detected with both anti-Bax antibody (Oncor) and anti-bcl-2 antibody (DAKO)immunohistochemically.RESULT : Apoptosis was 0.02% before irradiation, and it significantly increased up to 1.2% at 9Gy. The Dositive rate of Bax was 15% before irradiation, and it increased up to 60% at 9Gy. While that of bcl-2 was 15% before irradiation, and it increased to 25% at 9Gy which was no significant increase. Additionally, a significant correlation was observed between Bax expression and apoptosis of the tumor at 9Gy.CONCLUSION : Bax protein expression significantly correlates with radiation-induced apoptosis of cervical cancer.2 Correlation between p53, p21 protein expression and radiation-induced apoptosisCorrelation between p53, p21 tumor expression and radiation-induced apoptosis were investigated. Before irradiation, the mean AI of p53 and p21 were 6.60% and 9.24% respectively. Then after 9Gy the mean AI of p53 and p21 significantly increased to 13.9% and 18.0% respectively. A significant positive correlation appeared between p53 protein index and p21 protein index before irradiation. Mean AI, p53 index, and p21 index were 0.98%, 7.33% and 8.77% respectively. As for radiation-induced AI, a significant negative correlation was observed between both AI and p53 or p21. Moreover, a significant positive correlation was observed between both p53 and p21.CONCLOSION : The cascade of p53 and p21 act as apoptosis inhibitory action in cervical cancer.