Ontogeny and differentiation of oligodendrocytes in the central nervous system using the antibody recognizing immature oligodendrocytes.

用小鼠单克隆抗体（克隆4F 2和12 F7）免疫化学方法检测了大鼠中枢神经系统（包括前脑和脊髓）从胚胎早期到成年期发育过程中少突胶质细胞的个体发育和分化。在胚胎早期（E9-E10），在脑室和邻近脑室区的神经上皮中观察到对克隆4F 7抗体免疫反应的细胞。它们广泛分布于大脑和脊髓。4F 7阳性细胞未被识别未成熟神经元的TUJ 1抗体免疫染色，表明它们是产生少突胶质细胞的祖细胞，而不是神经元。随着发育，这些细胞从软脑膜下的脑室区开始分布于脑壁，出生后最好分布在白色物质中而不是灰质中。它们的过程在新生儿时期是树枝状的，在成年人精细的放射状过程被拉长到包裹轴突。在成人中，4F 7阳性细胞用几种寡神经胶质细胞标记物标记。在成年和老年的侧脑室背外侧角的脑室下区观察到它们，表明存在未成熟的少突胶质细胞。另一个克隆（12 F7）仅在出生后才在白色物质的髓鞘部分显示免疫反应性。在白色物质中，所有的髓鞘均未显示12 F7免疫反应性。

The ontogeny and differentiation of oligodendrocytes were examined immunohistochemically with the mouse monoclonal antibodies (clones 4F2 and 12F7) that were generated using a huge amount of homogeneous population of stage-specific immature oligodendrocytes as a immunogen, in the rat central nervous system including the prosencephalon and the spinal cord during the development from the early embryonal to the adult period. The cells immunoreactive for the antibody of clone 4F7 were observed in the neuroepithelium lining the ventricles and the neighboring ventricular zone in the early embryonal days (E9-E10). They were widely distributed through the brain and spinal cord. The 4F7-positive cells were not immunostained with the TUJ1 antibody that recognizes the immature neurons, suggesting that they are the progenitors to generate the oligodendrocytes, but not neurons. With development, these cells were located th roughout the wall of the brain from the ventricular zone beneath the pia mater, and were preferably in the white matter rather than the gray matter after birth. Their processes were arborized in the neonatal period, and in adults fine radiated processes were elongated to envelop the axons. The 4F7-positive cells were labeled with several oligodedroglial markers in adults. They were observed in the subventricular zone at the dorsolateral corner of the lateral ventricle in adults and senility, suggesting the presence of immature oligodendrocytes. Another clone (12F7) showed the immunoreactivity in the myelin part of the white matter only after birth. Within the white matter, all of the myelin did not show the 12F7 immunoreactivity.