A study of genetic management of fish population and genetic diversity used micro satellite DNA markers

在鱼类遗传多样性的研究实验中，对以下物种进行了检查，Ayu和Masu鲑鱼作为代表性的自然种群，锦鲤作为代表性的观赏鱼，冲浪鱼作为代表性的胎生鱼，孔雀鱼作为实验鱼，琉球Ayu和Matsukaws作为濒危物种。在这些物种中，利用微卫星DNA标记技术，对不同物种的遗传多样性进行了研究，并对遗传变异和遗传差异进行了分析微卫星DNA标记。根据平均杂合度计算的养殖群体的遗传多样性的减少被观察到作为近交的结果。从每个养殖群体和用于释放的种子群体中群体的有效大小的估计，通过对濒危物种种群的紧急程度进行评估，估计了近亲繁殖对种群的影响，并提出了补救措施。提出了根据亲本群体基因型数据模拟杂交试验，提出了濒危物种遗传修复和遗传多样性保护的方法。

Following species were examined for the research experiment of genetic divereity in fishes, Ayu and Masu salmon as representative natural population, Koi carp as representative ornamental fish, Surf fish as representative viviparous fish, Guppy as experimental fish, and Ryukyu-Ayu and Matsukaws as endangered species.In these species, microsatellite DNA markers were developed to evaluate genetic diversity.Genetic characteristics such as genetic variability and differences were measured in each species used microsatellite DNA markers.The reduction of genetic diversity calculated from average heterozygosity in cultured populations were observed as the results of inbreeding.From the estimation of effective size of population in each cultured population and seed population for release, the degree of emergency could be estimated in the population of the endangered species.The influence of inbreeding to the population were estimated and methods of remediation was proposed. From the simulation of cross experiment based on the genotype data of parent population, the methods for the genetic remediation in endangered species and conservation of genetic diversity were also proporsed.