Magnetic probe with ferrite couples to bioligands for diagnostic and clinical uses

1.新型医用铁氧体纳米珠的研制我们建立了在水溶液合成铁氧体（Fe_3O_（4+8））纳米珠（直径10-15 nm）的过程中将生理活性分子固定在纳米珠表面的技术。我们制备了医用磁性纳米微球，在其表面牢固地固定了胰蛋白酶、FITC-亲和素、抗癌药物、甲基丙烯酸缩水甘油酯（GMA）、氨基酸（如天冬氨酸）和类似结构的分子、聚丙烯酰氨基酸等生理活性分子.我们发现，在上述的菲尼特纳米珠的生理活性分子是强烈的耦合到铁氧体中间的氨基酸或类似结构的分子中包含的生理活性分子的S-H和COOH基团。我们将中间的氨基酸（和类似结构的分子）命名为连接器。我们还称之为医用纳米珠（具有生理活性分子， ...更多信息 由连接器介导的表面）“连接器结合的磁性（CBM）”纳米珠。此外，我们开发了CBM纳米珠，其表面涂覆有（1）连接体和（2）对生理活性分子的非特异性吸收表现出低敏感性的聚合物;在其表面上，我们可以通过生物化学技术容易地固定生理活性分子。人型抗体及新型铁氧体生物探针的制备我们将鼠单克隆抗体的恒定区替换为人IgG抗体的恒定区，制备了抗人结肠癌细胞的人型IgG抗体。固定在铁氧体珠上的人型IGG抗体选择性地与存在于人结肠癌细胞膜上的抗原反应，所述抗原通过用与荧光缀合的抗IGG抗体染色而可视化。这表明IGG抗体固定的铁氧体珠作为诊断磁性生物探针用于检测癌症。铁氧体纳米微球对高血压的功率吸收率我们发现，本研究制备的铁氧体纳米微球对交流磁场感应的比功率吸收率比常规铁氧体微球高1.4倍。这表明我们的铁氧体纳米珠对于应用于热疗是有利的。少

1. Development of novel medical ferrite Nano-BeadsWe established the technique to immobilize physiologically active molecules onto the surfaces of ferrite (Fe_3O_<4+8>) nano beads (10-15 run in diameter) during the beads are synthesized from an aqueous solution. We prepared medical magnetic nano beads on the surfaces of which we strongly immobilized such physiologically active molecules as Trypsin FITC-avidin, anti-cancer drugs, glycidyl methacrylate (GMA), amino acids (e.g. aspartic acid) and similar-strusture molecules, polyacryl amino acid, etc.2. We revealed that in the above-mentioned fenite nano beads the physiologically active molecules are strongly couples to ferrite intermediated by S-H and COOH groups of the amino acids or similar-structure molecules contained in the physiologically active molecules. We named the intermediating amino acids (and similar structured molecules) connectors. Also we called the medical nano beads (with physiologically active molecules coupled to the … More surfaces intermediated by the connectors) "Connectors-Bonded Magnetic (CBM)" nano beads. Furthermore, we developed CBM nano beads having surfaces coated with (1)connectors and (2)polymers exhibiting low sensitivity to non-specific absorption of physiologically active molecules ; on their surfaces we can easily immobilize physiologically active molecules by biochemical technique.3. Preparation of human-type antibody and novel ferrite bioprobesWe have prepared human-type IGG antibody to human colon cancer cells by replacing the constant region of mouse monoclonal IGG antibody with that of human IGG antibody. The human-type IGG antibody fixed on the ferrite beads selectively reacted with the antigens present on human colon cancer cell membrane, which were visualized by staining with anti-IGG antibody conjugated with fluorescence. This shows that die IGG antibody-fixed ferrite beads are utilized for detection of cancer as diagnostic magnetic bioprobes.4. Power absorption rate of ferrite nano beads for hyperthermiaWe found that the fenite nano beads prepared in this study have specific power absorption rate for AC magnetic field induction 1.4 times higher than that of conventional ferrite beads. This indicates that our ferrite nano beads are advantageous for applying to hyperthermia. Less