Melecular mechanism of cdholesterol hydroperoxides as indices membrane disorder and its application to forensic medicine

1.百草枯中毒通过分析胆固醇衍生的过氧化氢(7α-00H和7β-00H)作为脂质过氧化指标，研究百草枯诱导的体内氧化应激。百草枯(10 mg/kg)ip。对老鼠来说。在肾脏和肝脏中，脂质过氧化反应在2小时后开始增强。肾脏在第5天仍有增加。结论：1.小剂量急性百草枯处理大鼠肾脏病理改变可能反映了氧化应激的加重。酒精性肌病我们测试了这样的假设，即肌肉对酒精的敏感性可以在酒精暴露的最初阶段辨别出来。为了测试这一点，我们测量了比目鱼肌和足底肌肉中的胆固醇过氧化氢。2.5h后，乙醇仅在足底肌中增加7α00H和7β-00H。24小时后，两种过氧化氢在各肌肉中均升高。这些结果表明，II型肌肉比I型肌肉敏感。一氧化碳中毒我们测量了接受假手术(对照组)、低氧或吸入一氧化碳(1005ppm)90分钟后恢复48小时的大鼠大脑中的胆固醇过氧化氢。7α-00H和7β-00H在缺氧组明显降低，而CO组与对照组相比无明显变化。提示一氧化碳通过不依赖于低氧的脂质过氧化作用对脑组织造成损伤。

1. Paraquat poisoningThe in vivo paraquat-induced oxidative stress was studied by analyzing cholesterol-derived hydroperoxide (7α -00H and 7β -00H) as an index of lipid peroxidation. Paraquat (10mg/kg) was administered i.p. to rats. In kidney and liver, augumented lipid peroxidation was found at 2h. The increase remained in kidney 5 days. It seems to reflect greater oxidative stress in the pathology of kidney of rats treated with acute paraquat at low dose.2. Alcoholic myopathyWe tested the hypotheses that ethanol sensitivities of muscle can be discerned in the initial periods of ethanol exposure. To test this we measured cholesterol hydroperoxides in soleus and plantaris muscle. After 2.5 h, ethanol increased 7α 00H and 7β -00H in plantaris muscle alone. After 24 h both hydroperoxides wefe elevated in each muscle. These results suggest that Type II muscle is sensitive than Type I muscle.3. CO poisoningWe measured cholesterol hydroperoxides in the brains of rats that underwent either a sham operation (control), hypoxia, or CO inhalation (1005 ppm) for 90 min followed by 48 h of recovery. 7α-00H and 7β-00H were low in the hypoxia group, while these were unaltered in the CO group compared with the controls. It suggests that CO injures to the brain through lipid peroxidation independently of hypoxia.