Clinical application of Caftilage-derived Retinoic Acid-sensitive Protein

为了研究关节疾病与软骨源性视黄酸敏感蛋白（CD-RAP）的相关性，我们测量了骨关节炎（OA）和类风湿性关节炎（RA）患者滑液中- (SF)的浓度。OA组CD-RAP平均SF浓度明显高于RA组。轻度OA组CD-RAP明显高于中度和重度OA组。轻度RA组的CD-RAP也显著高于其他RA组，并随着疾病进展而降低。免疫组化研究显示，CD-RAP在新形成的纤维软骨软骨细胞细胞质中有表达。由于CD-RAP主要在年轻的增殖软骨细胞中产生，这些结果提示SF中CD-RAP的水平反映了关节软骨的重塑，有可能作为客观评价软骨细胞修复反应的标志物。为了定量测定人脑脊液中CD-RAP的含量并明确其性质，我们测定了脊髓疾病患者脑脊液中CD-RAP的表达。CD-RAP在颈椎病、腰椎管狭窄和腰椎间盘突出症患者中的浓度明显高于对照组。CD-RAP浓度在对照组中较低，而在导致椎管狭窄的脊柱疾病中则明显较高。CD-RAP在脑脊液中表达是神经结构损伤或受压的结果，可能是脊柱疾病的标志。为了研究CD-RAP在软骨肉瘤血清中的表达，我们测定了Sworm大鼠软骨肉瘤血清中CD-RAP的浓度。浓度随着肿瘤的进展而增加。肿瘤切除后，浓度降至正常范围。结果表明CD-RAP可能成为软骨肉瘤的潜在标志物。

To investigate the correlation between joint diseases and Cartilage-derived Retinoic Acid-sensitive Protein (CD-RAP), we measured the concentrations in synovial fluid- (SF) from osteoarthritis (OA) and rheumatoid arthritis (RA). The mean SF concentration of CD-RAP was significantly higher in OA than in RA. The CD-RAP in the mild OA group was significantly higher than in the moderate or severe OA groups. CD-RAP in the mild RA group was also significantly higher than in the other RA groups and decreased with disease progression. Immunohistochemical study showed that CD-RAP expression was observed in the cytoplasm of chondrocytes in newly formed fibrocartilage. As CD-RAP is mainly produced in young and proliferating chondrocytes, these results suggest that the level of CD-RAP in SF reflects remodeling of articular cartilage and has a possibility of a marker to objectively estimate the restorative reaction of chondrocytes.To quantify CD-RAP in human cerebrospinal fluid and to clarify its character, the expression was measured in cerebrospinal fluid from patients with spinal diseases. The concentrations of CD-RAP in cervical myelopathy, lumbar canal stenosis, and lumbar disc herniation were significantly higher than in the control group. The CD-RAP concentration was low in the control group, whereas it was significantly higher in spinal diseases that cause spinal stenosis. CD-RAP is expressed in cerebrospinal fluid as a result of damage to or stressing of neural structures and could be a marker for spinal diseases.To study the expression in serctm from chondrosarcoma, we measured CD-RAP concentration in serum of Sworm rat chondrosarcoma. The concentration increased with progression of the tumor. When the tumor was excised, the concentration went down to the normal range. The results show that CD-RAP could become a potential marker of chondrosarcoma.